This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2010-07-14    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
metallopeptidase activity	ISS: Inferred from Sequence or structural Similarity with EBI:Q92905 Assigned on 2010-07-14	Verma R, et al.  (2002) Role of Rpn11 metalloprotease in deubiquitination and degradation by the 26S proteasome. Science 298(5593):611-5	SGD
ubiquitin-specific protease activity	IMP: Inferred from Mutant Phenotype Assigned on 2010-07-14	Yao T and Cohen RE  (2002) A cryptic protease couples deubiquitination and degradation by the proteasome. Nature 419(6905):403-7	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-07-14	Verma R, et al.  (2002) Role of Rpn11 metalloprotease in deubiquitination and degradation by the 26S proteasome. Science 298(5593):611-5	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrial fission	IMP: Inferred from Mutant Phenotype Assigned on 2009-10-07	Hofmann L, et al.  (2009) A nonproteolytic proteasome activity controls organelle fission in yeast. J Cell Sci 122(Pt 20):3673-83	SGD
peroxisome fission	IMP: Inferred from Mutant Phenotype Assigned on 2009-10-07	Hofmann L, et al.  (2009) A nonproteolytic proteasome activity controls organelle fission in yeast. J Cell Sci 122(Pt 20):3673-83	SGD
proteasomal ubiquitin-dependent protein catabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2010-07-14	Verma R, et al.  (2002) Role of Rpn11 metalloprotease in deubiquitination and degradation by the 26S proteasome. Science 298(5593):611-5	SGD
protein deubiquitination	IMP: Inferred from Mutant Phenotype Assigned on 2010-07-14	Verma R, et al.  (2002) Role of Rpn11 metalloprotease in deubiquitination and degradation by the 26S proteasome. Science 298(5593):611-5	SGD
	IGI: Inferred from Genetic Interaction with SGD:UBP6 Assigned on 2010-07-14	Guterman A and Glickman MH  (2004) Complementary roles for Rpn11 and Ubp6 in deubiquitination and proteolysis by the proteasome. J Biol Chem 279(3):1729-38	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytosol	IDA: Inferred from Direct Assay Assigned on 2009-10-07	Hofmann L, et al.  (2009) A nonproteolytic proteasome activity controls organelle fission in yeast. J Cell Sci 122(Pt 20):3673-83	SGD
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2009-10-07	Hofmann L, et al.  (2009) A nonproteolytic proteasome activity controls organelle fission in yeast. J Cell Sci 122(Pt 20):3673-83	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2005-03-14	Wendler P, et al.  (2004) The bipartite nuclear localization sequence of Rpn2 is required for nuclear import of proteasomal base complexes via karyopherin alphabeta and proteasome functions. J Biol Chem 279(36):37751-62	SGD
proteasome regulatory particle, lid subcomplex	IDA: Inferred from Direct Assay Assigned on 2002-08-23	Fu H, et al.  (2001) Subunit interaction maps for the regulatory particle of the 26S proteasome and the COP9 signalosome. EMBO J 20(24):7096-107	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-05-26	Glickman MH, et al.  (1998) A subcomplex of the proteasome regulatory particle required for ubiquitin-conjugate degradation and related to the COP9-signalosome and eIF3. Cell 94(5):615-23	SGD
proteasome storage granule	IDA: Inferred from Direct Assay Assigned on 2008-06-25	Laporte D, et al.  (2008) Reversible cytoplasmic localization of the proteasome in quiescent yeast cells. J Cell Biol 181(5):737-45	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for RPN11

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Cellular Component

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
proteasome complex	IEA: Inferred from Electronic Annotation with EBI:KW-0647 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).