This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-02-14    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
lipid binding	IDA: Inferred from Direct Assay Assigned on 2011-02-14	Welker S, et al.  (2010) Hsp12 Is an Intrinsically Unstructured Stress Protein that Folds upon Membrane Association and Modulates Membrane Function. Mol Cell 39(4):507-520	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell adhesion	IDA: Inferred from Direct Assay Assigned on 2002-04-03	Zara S, et al.  (2002) HSP12 is essential for biofilm formation by a Sardinian wine strain of S. cerevisiae. Yeast 19(3):269-76	SGD
cellular response to heat	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-14	Welker S, et al.  (2010) Hsp12 Is an Intrinsically Unstructured Stress Protein that Folds upon Membrane Association and Modulates Membrane Function. Mol Cell 39(4):507-520	SGD
cellular response to osmotic stress	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-14	Welker S, et al.  (2010) Hsp12 Is an Intrinsically Unstructured Stress Protein that Folds upon Membrane Association and Modulates Membrane Function. Mol Cell 39(4):507-520	SGD
cellular response to oxidative stress	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-14	Welker S, et al.  (2010) Hsp12 Is an Intrinsically Unstructured Stress Protein that Folds upon Membrane Association and Modulates Membrane Function. Mol Cell 39(4):507-520	SGD
plasma membrane organization	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-14	Welker S, et al.  (2010) Hsp12 Is an Intrinsically Unstructured Stress Protein that Folds upon Membrane Association and Modulates Membrane Function. Mol Cell 39(4):507-520	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytosol	IDA: Inferred from Direct Assay Assigned on 2011-02-14	Welker S, et al.  (2010) Hsp12 Is an Intrinsically Unstructured Stress Protein that Folds upon Membrane Association and Modulates Membrane Function. Mol Cell 39(4):507-520	SGD
endosome	IDA: Inferred from Direct Assay Assigned on 2011-02-14	Welker S, et al.  (2010) Hsp12 Is an Intrinsically Unstructured Stress Protein that Folds upon Membrane Association and Modulates Membrane Function. Mol Cell 39(4):507-520	SGD
plasma membrane	IDA: Inferred from Direct Assay Assigned on 2011-02-14	Welker S, et al.  (2010) Hsp12 Is an Intrinsically Unstructured Stress Protein that Folds upon Membrane Association and Modulates Membrane Function. Mol Cell 39(4):507-520	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-07-02	Sales K, et al.  (2000) The LEA-like protein HSP 12 in Saccharomyces cerevisiae has a plasma membrane location and protects membranes against desiccation and ethanol-induced stress. Biochim Biophys Acta 1463(2):267-78	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
plasma membrane	IDA: Inferred from Direct Assay Assigned on 2009-04-29	Delom F, et al.  (2006) The plasma membrane proteome of Saccharomyces cerevisiae and its response to the antifungal calcofluor. Proteomics 6(10):3029-39	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
response to stress	IEA: Inferred from Electronic Annotation with EBI:IPR007250 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0346 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).