This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-02-07    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
mating-type alpha-factor pheromone receptor activity	IGI: Inferred from Genetic Interaction with SGD:GPA1, SGD:STE18, SGD:STE4 Assigned on 2005-05-12 IMP: Inferred from Mutant Phenotype Assigned on 2005-05-12	Dosil M, et al.  (1998) Dominant-negative mutations in the G-protein-coupled alpha-factor receptor map to the extracellular ends of the transmembrane segments. Mol Cell Biol 18(10):5981-91	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-07-07	Jenness DD, et al.  (1983) Binding of alpha-factor pheromone to yeast a cells: chemical and genetic evidence for an alpha-factor receptor. Cell 35(2 Pt 1):521-9	SGD
	ISS: Inferred from Sequence or structural Similarity Assigned on 2001-01-18	Burkholder AC and Hartwell LH  (1985) The yeast alpha-factor receptor: structural properties deduced from the sequence of the STE2 gene. Nucleic Acids Res 13(23):8463-75	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-05-12	Blumer KJ and Thorner J  (1990) Beta and gamma subunits of a yeast guanine nucleotide-binding protein are not essential for membrane association of the alpha subunit but are required for receptor coupling. Proc Natl Acad Sci U S A 87(11):4363-7	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell projection assembly	IMP: Inferred from Mutant Phenotype Assigned on 2003-02-07	Vallier LG, et al.  (2002) The alpha-factor receptor C-terminus is important for mating projection formation and orientation in Saccharomyces cerevisiae. Cell Motil Cytoskeleton 53(4):251-66	SGD
cytogamy	IMP: Inferred from Mutant Phenotype Assigned on 2007-07-12 IDA: Inferred from Direct Assay Assigned on 2007-07-12	Shi C, et al.  (2007) A role for a complex between activated G protein-coupled receptors in yeast cellular mating. Proc Natl Acad Sci U S A 104(13):5395-400	SGD
pheromone-dependent signal transduction involved in conjugation with cellular fusion	IMP: Inferred from Mutant Phenotype Assigned on 2007-07-12	Jenness DD, et al.  (1987) Saccharomyces cerevisiae mutants unresponsive to alpha-factor pheromone: alpha-factor binding and extragenic suppression. Mol Cell Biol 7(4):1311-9	SGD
protein homooligomerization	IPI: Inferred from Physical Interaction with SGD:STE2 Assigned on 2007-09-04	Gehret AU, et al.  (2006) Oligomerization of the yeast alpha-factor receptor: implications for dominant negative effects of mutant receptors. J Biol Chem 281(30):20698-714	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to plasma membrane	IDA: Inferred from Direct Assay Assigned on 2002-11-22	Blumer KJ, et al.  (1988) The STE2 gene product is the ligand-binding component of the alpha-factor receptor of Saccharomyces cerevisiae. J Biol Chem 263(22):10836-42	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	ISM: Inferred from Sequence Model Assigned on 2011-05-31	De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
G-protein coupled receptor activity	IEA: Inferred from Electronic Annotation with EBI:KW-0297 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mating-type factor pheromone receptor activity	IEA: Inferred from Electronic Annotation with EBI:IPR000366 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
signal transducer activity	IEA: Inferred from Electronic Annotation with EBI:KW-0807 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
G-protein coupled receptor signaling pathway	IEA: Inferred from Electronic Annotation with EBI:IPR000366 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0297 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
response to pheromone	IEA: Inferred from Electronic Annotation with EBI:KW-0589 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
signal transduction	IEA: Inferred from Electronic Annotation with EBI:KW-0807 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0812 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:IPR000366 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0162 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).