This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-10-05    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA-directed RNA polymerase activity	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Kelly JL, et al.  (1986) Isolation of the nuclear gene encoding a subunit of the yeast mitochondrial RNA polymerase. J Biol Chem 261(22):10348-51	SGD
	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Greenleaf AL, et al.  (1986) Yeast RPO41 gene product is required for transcription and maintenance of the mitochondrial genome. Proc Natl Acad Sci U S A 83(10):3391-4	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrial genome maintenance	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Greenleaf AL, et al.  (1986) Yeast RPO41 gene product is required for transcription and maintenance of the mitochondrial genome. Proc Natl Acad Sci U S A 83(10):3391-4	SGD
transcription from mitochondrial promoter	IDA: Inferred from Direct Assay Assigned on 2011-10-05	Tang GQ, et al.  (2011) Transcription factor-dependent DNA bending governs promoter recognition by the mitochondrial RNA polymerase. J Biol Chem 286(44):38805-13	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Greenleaf AL, et al.  (1986) Yeast RPO41 gene product is required for transcription and maintenance of the mitochondrial genome. Proc Natl Acad Sci U S A 83(10):3391-4	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrial DNA-directed RNA polymerase complex	IDA: Inferred from Direct Assay Assigned on 2008-02-13	Mangus DA, et al.  (1994) Release of the yeast mitochondrial RNA polymerase specificity factor from transcription complexes. J Biol Chem 269(42):26568-74	SGD
mitochondrial matrix	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Kelly JL, et al.  (1986) Isolation of the nuclear gene encoding a subunit of the yeast mitochondrial RNA polymerase. J Biol Chem 261(22):10348-51	SGD
	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Greenleaf AL, et al.  (1986) Yeast RPO41 gene product is required for transcription and maintenance of the mitochondrial genome. Proc Natl Acad Sci U S A 83(10):3391-4	SGD
mitochondrial nucleoid	IDA: Inferred from Direct Assay Assigned on 2005-04-01	Chen XJ, et al.  (2005) Aconitase couples metabolic regulation to mitochondrial DNA maintenance. Science 307(5710):714-7	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2012-12-12	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR002092 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
DNA-directed RNA polymerase activity	IEA: Inferred from Electronic Annotation with EBI:IPR024075, EBI:IPR002092 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0240 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with IUBMB:2.7.7.6 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
nucleotidyltransferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0548 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:IPR002092 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IEA: Inferred from Electronic Annotation with EBI:SL-0173 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0496 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).