This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2004-01-15    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
cyclin binding	IPI: Inferred from Physical Interaction with SGD:CLB2 Assigned on 2008-08-28	Kellogg DR, et al.  (1995) Members of the NAP/SET family of proteins interact specifically with B-type cyclins. J Cell Biol 130(3):661-73	SGD
histone binding	IDA: Inferred from Direct Assay Assigned on 2007-09-11	Luk E, et al.  (2007) Chz1, a Nuclear Chaperone for Histone H2AZ. Mol Cell 25(3):357-68	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-03-23	Noda M, et al.  (2011) Assembly states of the nucleosome assembly protein 1 (NAP-1) revealed by sedimentation velocity and non-denaturing MS. Biochem J 436(1):101-12	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
budding cell bud growth	IGI: Inferred from Genetic Interaction Assigned on 2004-01-15 IMP: Inferred from Mutant Phenotype Assigned on 2004-01-15	Shields CM, et al.  (2003) Saccharomyces cerevisiae Ats1p interacts with Nap1p, a cytoplasmic protein that controls bud morphogenesis. Curr Genet 44(4):184-94	SGD
M phase of mitotic cell cycle	IGI: Inferred from Genetic Interaction with SGD:CLB4, SGD:CLB3, SGD:CLB1 Assigned on 2007-02-16 IMP: Inferred from Mutant Phenotype Assigned on 2007-02-16	Kellogg DR and Murray AW  (1995) NAP1 acts with Clb1 to perform mitotic functions and to suppress polar bud growth in budding yeast. J Cell Biol 130(3):675-85	SGD
nucleosome assembly	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-01-30 IDA: Inferred from Direct Assay Assigned on 2002-01-30	Ishimi Y and Kikuchi A  (1991) Identification and molecular cloning of yeast homolog of nucleosome assembly protein I which facilitates nucleosome assembly in vitro. J Biol Chem 266(11):7025-9	SGD
nucleosome disassembly	IDA: Inferred from Direct Assay Assigned on 2008-09-10	Lorch Y, et al.  (2006) Chromatin remodeling by nucleosome disassembly in vitro. Proc Natl Acad Sci U S A 103(9):3090-3	SGD
positive regulation of transcription elongation from RNA polymerase II promoter	IDA: Inferred from Direct Assay Assigned on 2013-03-07	Kuryan BG, et al.  (2012) Histone density is maintained during transcription mediated by the chromatin remodeler RSC and histone chaperone NAP1 in vitro. Proc Natl Acad Sci U S A 109(6):1931-6	SGD
protein import into nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2008-08-28	Mosammaparast N, et al.  (2002) A role for nucleosome assembly protein 1 in the nuclear transport of histones H2A and H2B. EMBO J 21(23):6527-38	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2007-02-16	Kellogg DR, et al.  (1995) Members of the NAP/SET family of proteins interact specifically with B-type cyclins. J Cell Biol 130(3):661-73	SGD
colocalizes_with nuclear nucleosome	IDA: Inferred from Direct Assay Assigned on 2007-02-16	Fujii-Nakata T, et al.  (1992) Functional analysis of nucleosome assembly protein, NAP-1. The negatively charged COOH-terminal region is not necessary for the intrinsic assembly activity. J Biol Chem 267(29):20980-6	SGD
nucleus	IPI: Inferred from Physical Interaction Assigned on 2002-10-14	Shimizu Y, et al.  (2000) NBP1 (Nap1 binding protein 1), an essential gene for G2/M transition of Saccharomyces cerevisiae, encodes a protein of distinct sub-nuclear localization. Gene 246(1-2):395-404	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for NAP1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA binding	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleosome assembly	IEA: Inferred from Electronic Annotation with EBI:IPR002164 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular bud neck	IEA: Inferred from Electronic Annotation with EBI:SL-0029 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:IPR002164 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).