This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-02-10    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein binding	IPI: Inferred from Physical Interaction with SGD:MEX67 Assigned on 2011-02-10	Strasser K, et al.  (2000) Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export. J Cell Biol 150(4):695-706	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
poly(A)+ mRNA export from nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2002-09-16	Kadowaki T, et al.  (1994) Nuclear mRNA accumulation causes nucleolar fragmentation in yeast mtr2 mutant. Mol Biol Cell 5(11):1253-63	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2002-09-16 IPI: Inferred from Physical Interaction Assigned on 2002-09-16	Santos-Rosa H, et al.  (1998) Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores. Mol Cell Biol 18(11):6826-38	SGD
ribosomal large subunit export from nucleus	IGI: Inferred from Genetic Interaction with SGD:NMD3 Assigned on 2007-08-01 IMP: Inferred from Mutant Phenotype Assigned on 2007-08-01	Yao W, et al.  (2007) Nuclear Export of Ribosomal 60S Subunits by the General mRNA Export Receptor Mex67-Mtr2. Mol Cell 26(1):51-62	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nuclear pore	IDA: Inferred from Direct Assay Assigned on 2002-09-16	Santos-Rosa H, et al.  (1998) Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores. Mol Cell Biol 18(11):6826-38	SGD
nuclear RNA export factor complex	IPI: Inferred from Physical Interaction with SGD:MEX67 Assigned on 2007-08-01	Santos-Rosa H, et al.  (1998) Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores. Mol Cell Biol 18(11):6826-38	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	ISM: Inferred from Sequence Model Assigned on 2011-05-31	De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).