This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2005-10-06    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
GTP binding	ISS: Inferred from Sequence or structural Similarity with GenBank/EMBL/DDBJ:D21163 Assigned on 2005-05-27	Fabrizio P, et al.  (1997) An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2. EMBO J 16(13):4092-106	SGD
GTPase activity	ISS: Inferred from Sequence or structural Similarity with EBI:P13551, EBI:P13639 Assigned on 2005-05-27 IMP: Inferred from Mutant Phenotype Assigned on 2005-05-27	Fabrizio P, et al.  (1997) An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2. EMBO J 16(13):4092-106	SGD
U5 snRNA binding	IDA: Inferred from Direct Assay Assigned on 2005-10-06	Dix I, et al.  (1998) Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae. RNA 4(12):1675-86	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
assembly of spliceosomal tri-snRNP	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-24	Brenner TJ and Guthrie C  (2006) Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain. RNA 12(5):862-71	SGD
generation of catalytic spliceosome for first transesterification step	IGI: Inferred from Genetic Interaction with SGD:SAD1, SGD:BRR1, SGD:PRP28, SGD:SUB2, SGD:PRP8, SGD:BRR2, SGD:PRP24, SGD:SNU66, SGD:PRP2, SGD:PRP19 Assigned on 2005-10-04	Brenner TJ and Guthrie C  (2005) Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation. Genetics 170(3):1063-80	SGD
mRNA splicing, via spliceosome	IMP: Inferred from Mutant Phenotype Assigned on 2005-10-06	Fabrizio P, et al.  (1997) An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2. EMBO J 16(13):4092-106	SGD
spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-24	Brenner TJ and Guthrie C  (2006) Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain. RNA 12(5):862-71	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
U4/U6 x U5 tri-snRNP complex	IDA: Inferred from Direct Assay Assigned on 2005-09-26	Gottschalk A, et al.  (1999) Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP. EMBO J 18(16):4535-48	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-02-19	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
U5 snRNP	IDA: Inferred from Direct Assay Assigned on 2005-10-03	Stevens SW, et al.  (2001) Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae. RNA 7(11):1543-53	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for SNU114

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
GTP binding	IEA: Inferred from Electronic Annotation with EBI:IPR004161, EBI:IPR000795, EBI:IPR005517 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0342 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
GTPase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000795 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
GTP catabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR000795 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	GOC
mRNA processing	IEA: Inferred from Electronic Annotation with EBI:KW-0507 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
RNA splicing	IEA: Inferred from Electronic Annotation with EBI:KW-0508 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).