This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-10-18    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
core RNA polymerase I binding transcription factor activity	IGI: Inferred from Genetic Interaction with SGD:RPA43 Assigned on 2011-10-18 IDA: Inferred from Direct Assay Assigned on 2011-10-18 IPI: Inferred from Physical Interaction with SGD:RRN6, SGD:RPA43 Assigned on 2011-10-18	Peyroche G, et al.  (2000) The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3. EMBO J 19(20):5473-82	SGD
RNA polymerase I core binding	IDA: Inferred from Direct Assay Assigned on 2011-10-18 IPI: Inferred from Physical Interaction with SGD:RPA43 Assigned on 2011-10-18	Peyroche G, et al.  (2000) The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3. EMBO J 19(20):5473-82	SGD
RNA polymerase I transcription factor binding	IDA: Inferred from Direct Assay Assigned on 2011-10-18 IPI: Inferred from Physical Interaction with SGD:RRN6 Assigned on 2011-10-18	Peyroche G, et al.  (2000) The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3. EMBO J 19(20):5473-82	SGD
RNA polymerase I transcription factor binding transcription factor activity	IGI: Inferred from Genetic Interaction with SGD:RPA43 Assigned on 2011-10-18 IDA: Inferred from Direct Assay Assigned on 2011-10-18 IPI: Inferred from Physical Interaction with SGD:RRN6, SGD:RPA43 Assigned on 2011-10-18	Peyroche G, et al.  (2000) The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3. EMBO J 19(20):5473-82	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
transcription initiation from RNA polymerase I promoter for nuclear large rRNA transcript	IMP: Inferred from Mutant Phenotype Assigned on 2011-10-18	Yamamoto RT, et al.  (1996) RRN3 gene of Saccharomyces cerevisiae encodes an essential RNA polymerase I transcription factor which interacts with the polymerase independently of DNA template. EMBO J 15(15):3964-73	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleolus	IDA: Inferred from Direct Assay Assigned on 2009-05-17	Wnuk M, et al.  (2009) Rapid detection of yeast rRNA genes with primed in situ (PRINS) labeling. FEMS Yeast Res 9(4):634-40	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for RRN3

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).