This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2012-06-19    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleocytoplasmic transporter activity	IPI: Inferred from Physical Interaction with SGD:KAP95 Assigned on 2012-06-18	Patel SS, et al.  (2007) Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex. Cell 129(1):83-96	SGD
	IPI: Inferred from Physical Interaction with SGD:KAP95 Assigned on 2012-06-19	Jovanovic-Talisman T, et al.  (2009) Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Nature 457(7232):1023-7	SGD
	IPI: Inferred from Physical Interaction with SGD:KAP95 Assigned on 2012-06-18	Pyhtila B and Rexach M  (2003) A gradient of affinity for the karyopherin Kap95p along the yeast nuclear pore complex. J Biol Chem 278(43):42699-709	SGD
	IGI: Inferred from Genetic Interaction with SGD:NUP145 Assigned on 2012-06-07	Fiserova J, et al.  (2010) Facilitated transport and diffusion take distinct spatial routes through the nuclear pore complex. J Cell Sci 123(Pt 16):2773-80	SGD
	IPI: Inferred from Physical Interaction with SGD:ARX1 Assigned on 2012-06-18	Bradatsch B, et al.  (2007) Arx1 functions as an unorthodox nuclear export receptor for the 60S preribosomal subunit. Mol Cell 27(5):767-79	SGD
structural constituent of nuclear pore	IMP: Inferred from Mutant Phenotype Assigned on 2012-06-18 IPI: Inferred from Physical Interaction with SGD:NUP116, SGD:NUP100, SGD:NUP57, SGD:NUP49, SGD:NUP145, SGD:NUP42 Assigned on 2012-06-19	Patel SS, et al.  (2007) Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex. Cell 129(1):83-96	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-06-19	Jovanovic-Talisman T, et al.  (2009) Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Nature 457(7232):1023-7	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
mRNA export from nucleus	IPI: Inferred from Physical Interaction with SGD:MEX67 Assigned on 2011-05-23	Strawn LA, et al.  (2001) The GLFG regions of Nup116p and Nup100p serve as binding sites for both Kap95p and Mex67p at the nuclear pore complex. J Biol Chem 276(9):6445-52	SGD
mRNA export from nucleus in response to heat stress	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-23	Thomsen R, et al.  (2008) General, rapid, and transcription-dependent fragmentation of nucleolar antigens in S. cerevisiae mRNA export mutants. RNA 14(4):706-16	SGD
NLS-bearing substrate import into nucleus	IGI: Inferred from Genetic Interaction with SGD:NUP57, SGD:NUP145 Assigned on 2011-05-25	Strawn LA, et al.  (2004) Minimal nuclear pore complexes define FG repeat domains essential for transport. Nat Cell Biol 6(3):197-206	SGD
positive regulation of transcription, DNA-dependent	IGI: Inferred from Genetic Interaction with SGD:GCR1 Assigned on 2012-06-07	Menon BB, et al.  (2005) Reverse recruitment: the Nup84 nuclear pore subcomplex mediates Rap1/Gcr1/Gcr2 transcriptional activation. Proc Natl Acad Sci U S A 102(16):5749-54	SGD
posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-23	Light WH, et al.  (2010) Interaction of a DNA Zip code with the nuclear pore complex promotes H2A.Z incorporation and INO1 transcriptional memory. Mol Cell 40(1):112-25	SGD
protein import into nucleus	IGI: Inferred from Genetic Interaction with SGD:NUP145 Assigned on 2012-06-07	Fiserova J, et al.  (2010) Facilitated transport and diffusion take distinct spatial routes through the nuclear pore complex. J Cell Sci 123(Pt 16):2773-80	SGD
protein targeting to nuclear inner membrane	IGI: Inferred from Genetic Interaction with SGD:NUP57, SGD:NUP145 Assigned on 2012-06-11	Meinema AC, et al.  (2011) Long unfolded linkers facilitate membrane protein import through the nuclear pore complex. Science 333(6038):90-3	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nuclear pore	IDA: Inferred from Direct Assay Assigned on 2011-05-23	Wente SR and Blobel G  (1994) NUP145 encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure. J Cell Biol 125(5):955-69	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-03-31	Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51	SGD
nuclear pore central transport channel	IDA: Inferred from Direct Assay Assigned on 2012-06-27	Alber F, et al.  (2007) The molecular architecture of the nuclear pore complex. Nature 450(7170):695-701	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	ISM: Inferred from Sequence Model Assigned on 2011-05-31	De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
mRNA transport	IEA: Inferred from Electronic Annotation with EBI:KW-0509 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein transport	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:IPR007230 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0813, EBI:KW-0811 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nuclear membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0182 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
nuclear pore	IEA: Inferred from Electronic Annotation with EBI:IPR007230 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0185 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0906 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).