This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-12-15    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
contributes_to TBP-class protein binding	IDA: Inferred from Direct Assay Assigned on 2011-12-14	Stargell LA and Struhl K  (1995) The TBP-TFIIA interaction in the response to acidic activators in vivo. Science 269(5220):75-8	SGD
contributes_to TBP-class protein binding RNA polymerase II transcription factor activity	IDA: Inferred from Direct Assay Assigned on 2011-12-14	Stargell LA and Struhl K  (1995) The TBP-TFIIA interaction in the response to acidic activators in vivo. Science 269(5220):75-8	SGD
contributes_to TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-15 IDA: Inferred from Direct Assay Assigned on 2011-12-15	Warfield L, et al.  (2004) Positive and negative functions of the SAGA complex mediated through interaction of Spt8 with TBP and the N-terminal domain of TFIIA. Genes Dev 18(9):1022-34	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
RNA polymerase II transcriptional preinitiation complex assembly	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-15 IDA: Inferred from Direct Assay Assigned on 2011-12-15	Warfield L, et al.  (2004) Positive and negative functions of the SAGA complex mediated through interaction of Spt8 with TBP and the N-terminal domain of TFIIA. Genes Dev 18(9):1022-34	SGD
transcription initiation from RNA polymerase II promoter	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Kang JJ, et al.  (1995) Analysis of the yeast transcription factor TFIIA: distinct functional regions and a polymerase II-specific role in basal and activated transcription. Mol Cell Biol 15(3):1234-43	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
transcription factor TFIIA complex	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Kang JJ, et al.  (1995) Analysis of the yeast transcription factor TFIIA: distinct functional regions and a polymerase II-specific role in basal and activated transcription. Mol Cell Biol 15(3):1234-43	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for TOA2

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription initiation from RNA polymerase II promoter	IEA: Inferred from Electronic Annotation with EBI:IPR003194, EBI:IPR015872, EBI:IPR009088, EBI:IPR015871, EBI:IPR009083 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription factor TFIIA complex	IEA: Inferred from Electronic Annotation with EBI:IPR003194, EBI:IPR015872, EBI:IPR009088, EBI:IPR015871, EBI:IPR009083 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).