This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-10-28    Molecular Function | Biological Process

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
uridylyltransferase activity	IDA: Inferred from Direct Assay Assigned on 2012-12-11	Gunther Sillero MA, et al.  (2012) Methylenebisphosphonate and triphosphate derivatives of the mevalonate pathway are substrates of yeast UTP:glucose-1-phosphate uridylyltransferase. Biochimie 94(9):1871-5	SGD
UTP:glucose-1-phosphate uridylyltransferase activity	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-04-03 IMP: Inferred from Mutant Phenotype Assigned on 2002-04-03	Daran JM, et al.  (1995) Genetic and biochemical characterization of the UGP1 gene encoding the UDP-glucose pyrophosphorylase from Saccharomyces cerevisiae. Eur J Biochem 233(2):520-30	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
(1->6)-beta-D-glucan biosynthetic process	IMP: Inferred from Mutant Phenotype Assigned on 2007-06-11	Smith TL and Rutter J  (2007) Regulation of glucose partitioning by PAS kinase and Ugp1 phosphorylation. Mol Cell 26(4):491-9	SGD
glycogen biosynthetic process	IGI: Inferred from Genetic Interaction with SGD:PGM2, SGD:PGM1 Assigned on 2007-10-12 IMP: Inferred from Mutant Phenotype Assigned on 2007-10-12	Daran JM, et al.  (1997) Physiological and morphological effects of genetic alterations leading to a reduced synthesis of UDP-glucose in Saccharomyces cerevisiae. FEMS Microbiol Lett 153(1):89-96	SGD
trehalose biosynthetic process	IGI: Inferred from Genetic Interaction with SGD:PGM2, SGD:PGM1 Assigned on 2007-10-12	Daran JM, et al.  (1997) Physiological and morphological effects of genetic alterations leading to a reduced synthesis of UDP-glucose in Saccharomyces cerevisiae. FEMS Microbiol Lett 153(1):89-96	SGD
UDP-glucose metabolic process	IGI: Inferred from Genetic Interaction with SGD:PGM2, SGD:PGM1 Assigned on 2007-10-12 IMP: Inferred from Mutant Phenotype Assigned on 2007-10-12	Daran JM, et al.  (1997) Physiological and morphological effects of genetic alterations leading to a reduced synthesis of UDP-glucose in Saccharomyces cerevisiae. FEMS Microbiol Lett 153(1):89-96	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2002-04-03	Daran JM, et al.  (1995) Genetic and biochemical characterization of the UGP1 gene encoding the UDP-glucose pyrophosphorylase from Saccharomyces cerevisiae. Eur J Biochem 233(2):520-30	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
plasma membrane	IDA: Inferred from Direct Assay Assigned on 2009-04-29	Delom F, et al.  (2006) The plasma membrane proteome of Saccharomyces cerevisiae and its response to the antifungal calcofluor. Proteomics 6(10):3029-39	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotidyltransferase activity	IEA: Inferred from Electronic Annotation with EBI:IPR002618 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0548 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
UTP:glucose-1-phosphate uridylyltransferase activity	IEA: Inferred from Electronic Annotation with IUBMB:2.7.7.9 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR002618 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).