This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2004-08-13    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ubiquitin-protein ligase activity	TAS: Traceable Author Statement Assigned on 2001-01-18	Hochstrasser M  (1996) Ubiquitin-dependent protein degradation. Annu Rev Genet 30:405-39	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein monoubiquitination	TAS: Traceable Author Statement Assigned on 2001-01-18	Hochstrasser M  (1996) Ubiquitin-dependent protein degradation. Annu Rev Genet 30:405-39	SGD
protein polyubiquitination	TAS: Traceable Author Statement Assigned on 2001-01-18	Hochstrasser M  (1996) Ubiquitin-dependent protein degradation. Annu Rev Genet 30:405-39	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IPI: Inferred from Physical Interaction Assigned on 2002-12-09	Xie Y and Varshavsky A  (2000) Physical association of ubiquitin ligases and the 26S proteasome. Proc Natl Acad Sci U S A 97(6):2497-502	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2012-12-12	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-08-13	Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2012-12-12	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ligase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0436 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
ubiquitin-protein ligase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000569 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR11254_AN0 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular protein modification process	IEA: Inferred from Electronic Annotation with EBI:IPR000569 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
protein ubiquitination involved in ubiquitin-dependent protein catabolic process	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR11254_AN0 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
intracellular	IEA: Inferred from Electronic Annotation with EBI:IPR000569 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).