This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2004-06-17    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
LSU rRNA binding	ISA: Inferred from Sequence Alignment with SGD:RPP0 Assigned on 2010-02-05	Rodriguez-Mateos M, et al.  (2009) The amino terminal domain from Mrt4 protein can functionally replace the RNA binding domain of the ribosomal P0 protein. Nucleic Acids Res 37(11):3514-21	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
nuclear-transcribed mRNA catabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2004-06-17	Zuk D, et al.  (1999) Temperature-sensitive mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 genes result in defects in mRNA turnover. Genetics 153(1):35-47	SGD
ribosomal large subunit assembly	IGI: Inferred from Genetic Interaction with SGD:YVH1 Assigned on 2010-02-09 IMP: Inferred from Mutant Phenotype Assigned on 2010-02-09	Kemmler S, et al.  (2009) Yvh1 is required for a late maturation step in the 60S biogenesis pathway. J Cell Biol 186(6):863-80	SGD
ribosomal large subunit biogenesis	IMP: Inferred from Mutant Phenotype Assigned on 2002-09-27 IPI: Inferred from Physical Interaction Assigned on 2002-09-27	Harnpicharnchai P, et al.  (2001) Composition and functional characterization of yeast 66S ribosome assembly intermediates. Mol Cell 8(3):505-15	SGD
rRNA processing	IMP: Inferred from Mutant Phenotype Assigned on 2003-08-05	Peng WT, et al.  (2003) A panoramic view of yeast noncoding RNA processing. Cell 113(7):919-33	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleolus	IDA: Inferred from Direct Assay Assigned on 2010-02-05	Lo KY, et al.  (2009) Ribosome stalk assembly requires the dual-specificity phosphatase Yvh1 for the exchange of Mrt4 with P0. J Cell Biol 186(6):849-62	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-02-09	Kemmler S, et al.  (2009) Yvh1 is required for a late maturation step in the 60S biogenesis pathway. J Cell Biol 186(6):863-80	SGD
nucleoplasm	IDA: Inferred from Direct Assay Assigned on 2010-02-09	Kemmler S, et al.  (2009) Yvh1 is required for a late maturation step in the 60S biogenesis pathway. J Cell Biol 186(6):863-80	SGD
preribosome, large subunit precursor	IDA: Inferred from Direct Assay Assigned on 2009-01-09	Harnpicharnchai P, et al.  (2001) Composition and functional characterization of yeast 66S ribosome assembly intermediates. Mol Cell 8(3):505-15	SGD
colocalizes_with preribosome, large subunit precursor	IDA: Inferred from Direct Assay Assigned on 2010-02-09	Kemmler S, et al.  (2009) Yvh1 is required for a late maturation step in the 60S biogenesis pathway. J Cell Biol 186(6):863-80	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleolus	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
preribosome, large subunit precursor	IDA: Inferred from Direct Assay Assigned on 2008-08-25	Fuentes JL, et al.  (2007) In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1. Mol Genet Genomics 278(1):105-23	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
ribosome biogenesis	IEA: Inferred from Electronic Annotation with EBI:IPR001790 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0690 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
intracellular	IEA: Inferred from Electronic Annotation with EBI:IPR001790 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleolus	IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).