This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2012-06-18    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
adenyl-nucleotide exchange factor activity	IMP: Inferred from Mutant Phenotype Assigned on 2012-06-18 IDA: Inferred from Direct Assay Assigned on 2012-06-18	Noble KN, et al.  (2011) The Dbp5 cycle at the nuclear pore complex during mRNA export II: nucleotide cycling and mRNP remodeling by Dbp5 are controlled by Nup159 and Gle1. Genes Dev 25(10):1065-77	SGD
nucleocytoplasmic transporter activity	IPI: Inferred from Physical Interaction with SGD:KAP95 Assigned on 2012-06-18	Patel SS, et al.  (2007) Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex. Cell 129(1):83-96	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
ncRNA export from nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2011-06-20	Grosshans H, et al.  (2001) Biogenesis of the signal recognition particle (SRP) involves import of SRP proteins into the nucleolus, assembly with the SRP-RNA, and Xpo1p-mediated export. J Cell Biol 153(4):745-62	SGD
NLS-bearing substrate import into nucleus	IGI: Inferred from Genetic Interaction with SGD:NUP1, SGD:NUP60, SGD:NUP2, SGD:NUP42 Assigned on 2011-05-25	Strawn LA, et al.  (2004) Minimal nuclear pore complexes define FG repeat domains essential for transport. Nat Cell Biol 6(3):197-206	SGD
nuclear pore distribution	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-04	Gorsch LC, et al.  (1995) A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J Cell Biol 129(4):939-55	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-04	Del Priore V, et al.  (1997) A structure/function analysis of Rat7p/Nup159p, an essential nucleoporin of Saccharomyces cerevisiae. J Cell Sci 110 ( Pt 23):2987-99	SGD
poly(A)+ mRNA export from nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-04	Gorsch LC, et al.  (1995) A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J Cell Biol 129(4):939-55	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-04	Weirich CS, et al.  (2004) The N-terminal domain of Nup159 forms a beta-propeller that functions in mRNA export by tethering the helicase Dbp5 to the nuclear pore. Mol Cell 16(5):749-60	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-04	Del Priore V, et al.  (1997) A structure/function analysis of Rat7p/Nup159p, an essential nucleoporin of Saccharomyces cerevisiae. J Cell Sci 110 ( Pt 23):2987-99	SGD
	IGI: Inferred from Genetic Interaction with SGD:MEX67 Assigned on 2011-05-04	Strasser K, et al.  (2000) Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export. J Cell Biol 150(4):695-706	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-04	Sarkar S and Hopper AK  (1998) tRNA nuclear export in saccharomyces cerevisiae: in situ hybridization analysis. Mol Biol Cell 9(11):3041-55	SGD
protein export from nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-04	West M, et al.  (2007) Novel interaction of the 60S ribosomal subunit export adapter Nmd3 at the nuclear pore complex. J Biol Chem 282(19):14028-37	SGD
ribosomal large subunit export from nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2011-04-06	Stage-Zimmermann T, et al.  (2000) Factors affecting nuclear export of the 60S ribosomal subunit in vivo. Mol Biol Cell 11(11):3777-89	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-04	Gleizes PE, et al.  (2001) Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex. J Cell Biol 155(6):923-36	SGD
ribosomal small subunit export from nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-04	Gleizes PE, et al.  (2001) Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex. J Cell Biol 155(6):923-36	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nuclear pore	IDA: Inferred from Direct Assay Assigned on 2011-05-04	Gorsch LC, et al.  (1995) A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J Cell Biol 129(4):939-55	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-05-04	Kraemer DM, et al.  (1995) The essential yeast nucleoporin NUP159 is located on the cytoplasmic side of the nuclear pore complex and serves in karyopherin-mediated binding of transport substrate. J Biol Chem 270(32):19017-21	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-03-31	Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51	SGD
nuclear pore central transport channel	IDA: Inferred from Direct Assay Assigned on 2012-06-27	Alber F, et al.  (2007) The molecular architecture of the nuclear pore complex. Nature 450(7170):695-701	SGD
nuclear pore cytoplasmic filaments	IDA: Inferred from Direct Assay Assigned on 2012-06-27	Kiseleva E, et al.  (2004) Yeast nuclear pore complexes have a cytoplasmic ring and internal filaments. J Struct Biol 145(3):272-88	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-06-27	Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	ISM: Inferred from Sequence Model Assigned on 2011-05-31	De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
mRNA transport	IEA: Inferred from Electronic Annotation with EBI:KW-0509 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein transport	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813, EBI:KW-0811 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nuclear membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0182 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
nuclear pore	IEA: Inferred from Electronic Annotation with EBI:SL-0185 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0906 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).