This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2010-01-19    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
contributes_to DNA-directed RNA polymerase activity	IDA: Inferred from Direct Assay Assigned on 2010-01-19	Christie KR, et al.  (1994) Purified yeast RNA polymerase II reads through intrinsic blocks to elongation in response to the yeast TFIIS analogue, P37. J Biol Chem 269(2):936-43	SGD
contributes_to RNA-directed RNA polymerase activity	IDA: Inferred from Direct Assay Assigned on 2011-04-01	Lehmann E, et al.  (2007) Molecular basis of RNA-dependent RNA polymerase II activity. Nature 450(7168):445-9	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
termination of RNA polymerase II transcription	IMP: Inferred from Mutant Phenotype Assigned on 2010-01-19	Steinmetz EJ, et al.  (2006) cis- and trans-Acting determinants of transcription termination by yeast RNA polymerase II. Mol Cell Biol 26(7):2688-96	SGD
transcription from RNA polymerase II promoter	IMP: Inferred from Mutant Phenotype Assigned on 2010-01-19	Kolodziej P and Young RA  (1989) RNA polymerase II subunit RPB3 is an essential component of the mRNA transcription apparatus. Mol Cell Biol 9(12):5387-94	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA-directed RNA polymerase II, core complex	IDA: Inferred from Direct Assay Assigned on 2010-01-15	Sayre MH, et al.  (1992) Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J Biol Chem 267(32):23376-82	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	Woychik NA, et al.  (1990) Subunits shared by eukaryotic nuclear RNA polymerases. Genes Dev 4(3):313-23	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	Kolodziej PA, et al.  (1990) RNA polymerase II subunit composition, stoichiometry, and phosphorylation. Mol Cell Biol 10(5):1915-20	SGD
nucleoplasm	IDA: Inferred from Direct Assay Assigned on 2008-10-30	Bouchoux C, et al.  (2004) CTD kinase I is involved in RNA polymerase I transcription. Nucleic Acids Res 32(19):5851-60	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for RPB3

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR001514, EBI:IPR009025 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
DNA-directed RNA polymerase activity	IEA: Inferred from Electronic Annotation with EBI:IPR011262, EBI:IPR011261, EBI:IPR001514, EBI:IPR011263, EBI:IPR009025 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0240 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein dimerization activity	IEA: Inferred from Electronic Annotation with EBI:IPR011262, EBI:IPR011261 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:IPR011262, EBI:IPR011261, EBI:IPR011263, EBI:IPR001514, EBI:IPR009025 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).