This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2004-02-03    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
mannose binding	IMP: Inferred from Mutant Phenotype Assigned on 2004-02-03	Stratford M  (1989) Evidence for two mechanisms of flocculation in Saccharomyces cerevisiae. Yeast 5 Spec No:S441-5	SGD
	ISS: Inferred from Sequence or structural Similarity with SGD:FLO1 Assigned on 2004-02-03	Teunissen AW, et al.  (1995) Localization of the dominant flocculation genes FLO5 and FLO8 of Saccharomyces cerevisiae. Yeast 11(8):735-45	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell-substrate adhesion	IMP: Inferred from Mutant Phenotype Assigned on 2012-03-01	Van Mulders SE, et al.  (2009) Phenotypic diversity of Flo protein family-mediated adhesion in Saccharomyces cerevisiae. FEMS Yeast Res 9(2):178-90	SGD
flocculation	IMP: Inferred from Mutant Phenotype Assigned on 2009-05-10	Nonklang S, et al.  (2009) Construction of flocculent Kluyveromyces marxianus strains suitable for high-temperature ethanol fermentation. Biosci Biotechnol Biochem 73(5):1090-5	SGD
flocculation via cell wall protein-carbohydrate interaction	IMP: Inferred from Mutant Phenotype Assigned on 2012-03-01	Van Mulders SE, et al.  (2009) Phenotypic diversity of Flo protein family-mediated adhesion in Saccharomyces cerevisiae. FEMS Yeast Res 9(2):178-90	SGD
	IGI: Inferred from Genetic Interaction Assigned on 2002-12-19	Bidard F, et al.  (1994) Cloning and analysis of a FLO5 flocculation gene from S. cerevisiae. Curr Genet 25(3):196-201	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
fungal-type cell wall	TAS: Traceable Author Statement Assigned on 2002-10-17	Bossier P, et al.  (1997) Constitutive flocculation in Saccharomyces cerevisiae through overexpression of the GTS1 gene, coding for a 'Glo'-type Zn-finger-containing protein. Yeast 13(8):717-25	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-01-30	Bony M, et al.  (1998) Distribution of the flocculation protein, flop, at the cell surface during yeast growth: the availability of flop determines the flocculation level. Yeast 14(1):25-35	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for FLO5

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
flocculation	IEA: Inferred from Electronic Annotation with EBI:IPR001389 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
anchored to membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0336 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cell wall	IEA: Inferred from Electronic Annotation with EBI:SL-0041 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0134 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
extracellular region	IEA: Inferred from Electronic Annotation with EBI:KW-0964 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0162 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).