This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-10-28    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein serine/threonine kinase activity	IGI: Inferred from Genetic Interaction Assigned on 2002-11-11 ISS: Inferred from Sequence or structural Similarity Assigned on 2002-11-11	Toda T, et al.  (1988) SCH9, a gene of Saccharomyces cerevisiae that encodes a protein distinct from, but functionally and structurally related to, cAMP-dependent protein kinase catalytic subunits. Genes Dev 2(5):517-27	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
age-dependent response to oxidative stress involved in chronological cell aging	IMP: Inferred from Mutant Phenotype Assigned on 2003-08-14	Fabrizio P, et al.  (2001) Regulation of longevity and stress resistance by Sch9 in yeast. Science 292(5515):288-90	SGD
positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter	IMP: Inferred from Mutant Phenotype Assigned on 2012-03-29	Jorgensen P, et al.  (2004) A dynamic transcriptional network communicates growth potential to ribosome synthesis and critical cell size. Genes Dev 18(20):2491-505	SGD
positive regulation of transcription from RNA polymerase I promoter	IGI: Inferred from Genetic Interaction with SGD:TAP42 Assigned on 2009-09-01 IMP: Inferred from Mutant Phenotype Assigned on 2009-09-01	Huber A, et al.  (2009) Characterization of the rapamycin-sensitive phosphoproteome reveals that Sch9 is a central coordinator of protein synthesis. Genes Dev 23(16):1929-43	SGD
positive regulation of transcription from RNA polymerase III promoter	IGI: Inferred from Genetic Interaction with SGD:MAF1 Assigned on 2009-09-01 IMP: Inferred from Mutant Phenotype Assigned on 2009-09-01	Huber A, et al.  (2009) Characterization of the rapamycin-sensitive phosphoproteome reveals that Sch9 is a central coordinator of protein synthesis. Genes Dev 23(16):1929-43	SGD
protein phosphorylation	IGI: Inferred from Genetic Interaction Assigned on 2002-11-11 ISS: Inferred from Sequence or structural Similarity Assigned on 2002-11-11	Toda T, et al.  (1988) SCH9, a gene of Saccharomyces cerevisiae that encodes a protein distinct from, but functionally and structurally related to, cAMP-dependent protein kinase catalytic subunits. Genes Dev 2(5):517-27	SGD
regulation of protein localization	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-01	Huber A, et al.  (2009) Characterization of the rapamycin-sensitive phosphoproteome reveals that Sch9 is a central coordinator of protein synthesis. Genes Dev 23(16):1929-43	SGD
regulation of response to osmotic stress	IMP: Inferred from Mutant Phenotype Assigned on 2007-11-05	Pascual-Ahuir A and Proft M  (2007) The Sch9 kinase is a chromatin-associated transcriptional activator of osmostress-responsive genes. EMBO J 26(13):3098-108	SGD
regulation of transcription from RNA polymerase II promoter in response to oxidative stress	IMP: Inferred from Mutant Phenotype Assigned on 2007-11-05	Pascual-Ahuir A and Proft M  (2007) The Sch9 kinase is a chromatin-associated transcriptional activator of osmostress-responsive genes. EMBO J 26(13):3098-108	SGD
replicative cell aging	IMP: Inferred from Mutant Phenotype Assigned on 2005-03-14	Kaeberlein M, et al.  (2005) Genes determining yeast replicative life span in a long-lived genetic background. Mech Ageing Dev 126(4):491-504	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
colocalizes_with chromatin	IDA: Inferred from Direct Assay Assigned on 2007-11-05	Pascual-Ahuir A and Proft M  (2007) The Sch9 kinase is a chromatin-associated transcriptional activator of osmostress-responsive genes. EMBO J 26(13):3098-108	SGD
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2010-02-08	Patury S, et al.  (2009) Conditional nuclear import and export of yeast proteins using a chemical inducer of dimerization. Cell Biochem Biophys 53(3):127-34	SGD
fungal-type vacuole membrane	IDA: Inferred from Direct Assay Assigned on 2007-09-06	Urban J, et al.  (2007) Sch9 is a major target of TORC1 in Saccharomyces cerevisiae. Mol Cell 26(5):663-74	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-10-28	Jorgensen P, et al.  (2004) A dynamic transcriptional network communicates growth potential to ribosome synthesis and critical cell size. Genes Dev 18(20):2491-505	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2004-10-28	Jorgensen P, et al.  (2004) A dynamic transcriptional network communicates growth potential to ribosome synthesis and critical cell size. Genes Dev 18(20):2491-505	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Biological Process | Cellular Component

High-throughput Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
regulation of cell size	IMP: Inferred from Mutant Phenotype Assigned on 2006-07-14	Jorgensen P, et al.  (2002) Systematic identification of pathways that couple cell growth and division in yeast. Science 297(5580):395-400	SGD

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
fungal-type vacuole membrane	IDA: Inferred from Direct Assay Assigned on 2012-12-12	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:IPR017441, EBI:IPR000719, EBI:IPR002290, EBI:IPR017892, EBI:IPR000961 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
kinase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0418 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein kinase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR002290 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
protein serine/threonine kinase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000961, EBI:IPR017892, EBI:IPR008271 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0723 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity, transferring phosphorus-containing groups	IEA: Inferred from Electronic Annotation with EBI:IPR011009 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
phosphorylation	IEA: Inferred from Electronic Annotation with EBI:KW-0418 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein phosphorylation	IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR002290, EBI:IPR017892, EBI:IPR008271, EBI:IPR000961 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).