This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2004-08-03    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin binding	IDA: Inferred from Direct Assay Assigned on 2013-02-12	Huo L, et al.  (2012) The Rix1 (Ipi1p-2p-3p) complex is a critical determinant of DNA replication licensing independent of their roles in ribosome biogenesis. Cell Cycle 11(7):1325-39	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
pre-replicative complex assembly	IMP: Inferred from Mutant Phenotype Assigned on 2013-02-12	Huo L, et al.  (2012) The Rix1 (Ipi1p-2p-3p) complex is a critical determinant of DNA replication licensing independent of their roles in ribosome biogenesis. Cell Cycle 11(7):1325-39	SGD
regulation of DNA-dependent DNA replication initiation	IMP: Inferred from Mutant Phenotype Assigned on 2013-02-12	Huo L, et al.  (2012) The Rix1 (Ipi1p-2p-3p) complex is a critical determinant of DNA replication licensing independent of their roles in ribosome biogenesis. Cell Cycle 11(7):1325-39	SGD
ribosomal large subunit assembly	IMP: Inferred from Mutant Phenotype Assigned on 2005-03-10	Galani K, et al.  (2004) Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits. J Biol Chem 279(53):55411-8	SGD
ribosomal subunit export from nucleus	TAS: Traceable Author Statement Assigned on 2003-06-24 IPI: Inferred from Physical Interaction Assigned on 2003-06-24	Nissan TA, et al.  (2002) 60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm. EMBO J 21(20):5539-47	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2003-06-24	Gadal O, et al.  (2001) Nuclear export of 60s ribosomal subunits depends on Xpo1p and requires a nuclear export sequence-containing factor, Nmd3p, that associates with the large subunit protein Rpl10p. Mol Cell Biol 21(10):3405-15	SGD
rRNA processing	IMP: Inferred from Mutant Phenotype Assigned on 2004-08-03	Krogan NJ, et al.  (2004) High-definition macromolecular composition of yeast RNA-processing complexes. Mol Cell 13(2):225-39	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleoplasm	IDA: Inferred from Direct Assay Assigned on 2005-03-10	Galani K, et al.  (2004) Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits. J Biol Chem 279(53):55411-8	SGD
	IDA: Inferred from Direct Assay Assigned on 2003-06-24	Nissan TA, et al.  (2002) 60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm. EMBO J 21(20):5539-47	SGD
Rix1 complex	IDA: Inferred from Direct Assay Assigned on 2013-02-12	Krogan NJ, et al.  (2004) High-definition macromolecular composition of yeast RNA-processing complexes. Mol Cell 13(2):225-39	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IDA: Inferred from Direct Assay Assigned on 2004-01-05	Hazbun TR, et al.  (2003) Assigning function to yeast proteins by integration of technologies. Mol Cell 12(6):1353-65	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
ribosome biogenesis	IEA: Inferred from Electronic Annotation with EBI:KW-0690 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
rRNA processing	IEA: Inferred from Electronic Annotation with EBI:KW-0698 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).