This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2005-01-03    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein serine/threonine kinase activity	IDA: Inferred from Direct Assay Assigned on 2007-07-25	Robinson LC, et al.  (1993) Casein kinase I-like protein kinases encoded by YCK1 and YCK2 are required for yeast morphogenesis. Mol Cell Biol 13(5):2870-81	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell morphogenesis	IGI: Inferred from Genetic Interaction Assigned on 2003-01-31	Robinson LC, et al.  (1999) The Yck2 yeast casein kinase 1 isoform shows cell cycle-specific localization to sites of polarized growth and is required for proper septin organization. Mol Biol Cell 10(4):1077-92	SGD
cytokinesis	IGI: Inferred from Genetic Interaction Assigned on 2003-01-31	Robinson LC, et al.  (1999) The Yck2 yeast casein kinase 1 isoform shows cell cycle-specific localization to sites of polarized growth and is required for proper septin organization. Mol Biol Cell 10(4):1077-92	SGD
endocytosis	IGI: Inferred from Genetic Interaction Assigned on 2003-01-31	Robinson LC, et al.  (1999) The Yck2 yeast casein kinase 1 isoform shows cell cycle-specific localization to sites of polarized growth and is required for proper septin organization. Mol Biol Cell 10(4):1077-92	SGD
protein phosphorylation	ISS: Inferred from Sequence or structural Similarity Assigned on 2001-01-18 IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Robinson LC, et al.  (1992) Yeast casein kinase I homologues: an essential gene pair. Proc Natl Acad Sci U S A 89(1):28-32	SGD
response to glucose stimulus	IMP: Inferred from Mutant Phenotype Assigned on 2004-07-09	Moriya H and Johnston M  (2004) Glucose sensing and signaling in Saccharomyces cerevisiae through the Rgt2 glucose sensor and casein kinase I. Proc Natl Acad Sci U S A 101(6):1572-7	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
plasma membrane	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Vancura A, et al.  (1994) A prenylation motif is required for plasma membrane localization and biochemical function of casein kinase I in budding yeast. J Biol Chem 269(30):19271-8	SGD
	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Wang PC, et al.  (1992) Two genes in Saccharomyces cerevisiae encode a membrane-bound form of casein kinase-1. Mol Biol Cell 3(3):275-86	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Molecular Function | Biological Process | Cellular Component

High-throughput Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein kinase activity	IDA: Inferred from Direct Assay Assigned on 2012-05-04	Ptacek J, et al.  (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84	SGD

High-throughput Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein phosphorylation	IDA: Inferred from Direct Assay Assigned on 2012-05-04	Ptacek J, et al.  (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84	SGD

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
endoplasmic reticulum	IDA: Inferred from Direct Assay Assigned on 2002-05-07	Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19	SGD
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD
plasma membrane	IDA: Inferred from Direct Assay Assigned on 2009-04-29	Delom F, et al.  (2006) The plasma membrane proteome of Saccharomyces cerevisiae and its response to the antifungal calcofluor. Proteomics 6(10):3029-39	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:IPR017441, EBI:IPR000719 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
kinase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0418 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein kinase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000719 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
protein serine/threonine kinase activity	IEA: Inferred from Electronic Annotation with EBI:IPR008271 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0723 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity, transferring phosphorus-containing groups	IEA: Inferred from Electronic Annotation with EBI:IPR011009 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
phosphorylation	IEA: Inferred from Electronic Annotation with EBI:KW-0418 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein phosphorylation	IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR008271 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
plasma membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0039 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-1003 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).