This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2007-10-01    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA replication origin binding	IDA: Inferred from Direct Assay Assigned on 2007-08-24	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin silencing at silent mating-type cassette	IDA: Inferred from Direct Assay Assigned on 2007-09-26	Palacios DeBeer MA, et al.  (2003) Differential DNA affinity specifies roles for the origin recognition complex in budding yeast heterochromatin. Genes Dev 17(15):1817-22	SGD
DNA replication initiation	IMP: Inferred from Mutant Phenotype Assigned on 2010-05-14	Semple JW, et al.  (2006) An essential role for Orc6 in DNA replication through maintenance of pre-replicative complexes. EMBO J 25(21):5150-8	SGD
nuclear pre-replicative complex assembly	IDA: Inferred from Direct Assay Assigned on 2007-08-23	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA replication preinitiation complex	IDA: Inferred from Direct Assay Assigned on 2007-08-02	Zou L and Stillman B  (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6	SGD
nuclear origin of replication recognition complex	IMP: Inferred from Mutant Phenotype Assigned on 2007-01-31 IDA: Inferred from Direct Assay Assigned on 2007-01-31	Lee DG and Bell SP  (1997) Architecture of the yeast origin recognition complex bound to origins of DNA replication. Mol Cell Biol 17(12):7159-68	SGD
nuclear pre-replicative complex	IDA: Inferred from Direct Assay Assigned on 2007-08-02	Aparicio OM, et al.  (1997) Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase. Cell 91(1):59-69	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-23	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for ORC6

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR008721 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA replication	IEA: Inferred from Electronic Annotation with EBI:IPR008721 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0235 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nuclear origin of replication recognition complex	IEA: Inferred from Electronic Annotation with EBI:IPR008721 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).