This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-02-19    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
rRNA primary transcript binding	IPI: Inferred from Physical Interaction Assigned on 2003-02-19	Wehner KA and Baserga SJ  (2002) The sigma(70)-like motif: a eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis. Mol Cell 9(2):329-39	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IMP: Inferred from Mutant Phenotype Assigned on 2007-07-27	Wehner KA and Baserga SJ  (2002) The sigma(70)-like motif: a eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis. Mol Cell 9(2):329-39	SGD
maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IMP: Inferred from Mutant Phenotype Assigned on 2007-07-27	Wehner KA and Baserga SJ  (2002) The sigma(70)-like motif: a eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis. Mol Cell 9(2):329-39	SGD
ribosomal large subunit assembly	IPI: Inferred from Physical Interaction Assigned on 2002-08-26	Fatica A, et al.  (2002) Ssf1p prevents premature processing of an early pre-60S ribosomal particle. Mol Cell 9(2):341-51	SGD
ribosomal large subunit export from nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2009-05-01	Caesar S, et al.  (2006) Kap120 functions as a nuclear import receptor for ribosome assembly factor Rpf1 in yeast. Mol Cell Biol 26(8):3170-80	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleolus	IPI: Inferred from Physical Interaction Assigned on 2002-08-26	Fatica A, et al.  (2002) Ssf1p prevents premature processing of an early pre-60S ribosomal particle. Mol Cell 9(2):341-51	SGD
	IDA: Inferred from Direct Assay Assigned on 2003-02-19	Wehner KA and Baserga SJ  (2002) The sigma(70)-like motif: a eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis. Mol Cell 9(2):329-39	SGD
preribosome, large subunit precursor	IDA: Inferred from Direct Assay Assigned on 2009-01-09	Harnpicharnchai P, et al.  (2001) Composition and functional characterization of yeast 66S ribosome assembly intermediates. Mol Cell 8(3):505-15	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
preribosome, large subunit precursor	IDA: Inferred from Direct Assay Assigned on 2008-08-25	Fuentes JL, et al.  (2007) In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1. Mol Genet Genomics 278(1):105-23	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
RNA binding	IEA: Inferred from Electronic Annotation with EBI:KW-0694 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
rRNA binding	IEA: Inferred from Electronic Annotation with EBI:KW-0699 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
ribosome biogenesis	IEA: Inferred from Electronic Annotation with EBI:KW-0690 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
rRNA processing	IEA: Inferred from Electronic Annotation with EBI:KW-0698 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleolus	IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).