This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2010-05-17    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
antioxidant activity	IDA: Inferred from Direct Assay Assigned on 2010-05-17	Tamai KT, et al.  (1993) Yeast and mammalian metallothioneins functionally substitute for yeast copper-zinc superoxide dismutase. Proc Natl Acad Sci U S A 90(17):8013-7	SGD
cadmium ion binding	IDA: Inferred from Direct Assay Assigned on 2010-05-17	Winge DR, et al.  (1985) Yeast metallothionein. Sequence and metal-binding properties. J Biol Chem 260(27):14464-70	SGD
copper ion binding	IDA: Inferred from Direct Assay Assigned on 2002-07-20	Winge DR, et al.  (1985) Yeast metallothionein. Sequence and metal-binding properties. J Biol Chem 260(27):14464-70	SGD
superoxide dismutase activity	IMP: Inferred from Mutant Phenotype Assigned on 2010-05-17	Tamai KT, et al.  (1993) Yeast and mammalian metallothioneins functionally substitute for yeast copper-zinc superoxide dismutase. Proc Natl Acad Sci U S A 90(17):8013-7	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
detoxification of cadmium ion	IMP: Inferred from Mutant Phenotype Assigned on 2010-05-17	Ecker DJ, et al.  (1986) Yeast metallothionein function in metal ion detoxification. J Biol Chem 261(36):16895-900	SGD
detoxification of copper ion	IMP: Inferred from Mutant Phenotype Assigned on 2010-05-17	Ecker DJ, et al.  (1986) Yeast metallothionein function in metal ion detoxification. J Biol Chem 261(36):16895-900	SGD
removal of superoxide radicals	IGI: Inferred from Genetic Interaction with SGD:SOD1 Assigned on 2010-05-17 IDA: Inferred from Direct Assay Assigned on 2010-05-17	Tamai KT, et al.  (1993) Yeast and mammalian metallothioneins functionally substitute for yeast copper-zinc superoxide dismutase. Proc Natl Acad Sci U S A 90(17):8013-7	SGD
response to copper ion	IMP: Inferred from Mutant Phenotype Assigned on 2002-11-07	Butt TR, et al.  (1984) Cloning and expression of a yeast copper metallothionein gene. Gene 27(1):23-33	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytosol	IDA: Inferred from Direct Assay Assigned on 2002-07-22	Wright CF, et al.  (1987) Structural and functional studies of the amino terminus of yeast metallothionein. J Biol Chem 262(27):12912-9	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for CUP1-1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:IPR017854 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0480, EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).