This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-02-28    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
superoxide dismutase activity	IDA: Inferred from Direct Assay Assigned on 2002-10-01	Ravindranath SD and Fridovich I  (1975) Isolation and characterization of a manganese-containing superoxide dismutase from yeast. J Biol Chem 250(15):6107-12	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-05-03	Luk E, et al.  (2005) Manganese activation of superoxide dismutase 2 in the mitochondria of Saccharomyces cerevisiae. J Biol Chem 280(24):22715-20	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
age-dependent response to oxidative stress involved in chronological cell aging	IMP: Inferred from Mutant Phenotype Assigned on 2004-10-25	Fabrizio P, et al.  (2001) Regulation of longevity and stress resistance by Sch9 in yeast. Science 292(5515):288-90	SGD
age-dependent response to reactive oxygen species involved in chronological cell aging	IMP: Inferred from Mutant Phenotype Assigned on 2005-03-23	Longo VD, et al.  (1999) Mitochondrial superoxide decreases yeast survival in stationary phase. Arch Biochem Biophys 365(1):131-42	SGD
reactive oxygen species metabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-28	van Loon AP, et al.  (1986) A yeast mutant lacking mitochondrial manganese-superoxide dismutase is hypersensitive to oxygen. Proc Natl Acad Sci U S A 83(11):3820-4	SGD
replicative cell aging	IMP: Inferred from Mutant Phenotype Assigned on 2004-10-25	Fabrizio P, et al.  (2004) Chronological aging-independent replicative life span regulation by Msn2/Msn4 and Sod2 in Saccharomyces cerevisiae. FEBS Lett 557(1-3):136-42	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrial matrix	IDA: Inferred from Direct Assay Assigned on 2002-10-01	Ravindranath SD and Fridovich I  (1975) Isolation and characterization of a manganese-containing superoxide dismutase from yeast. J Biol Chem 250(15):6107-12	SGD
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2005-05-03	Luk E, et al.  (2005) Manganese activation of superoxide dismutase 2 in the mitochondria of Saccharomyces cerevisiae. J Biol Chem 280(24):22715-20	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2002-05-07	Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19	SGD
	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-09-24	Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:IPR019831, EBI:IPR001189, EBI:IPR019832, EBI:IPR019833 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
oxidoreductase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0560 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
superoxide dismutase activity	IEA: Inferred from Electronic Annotation with EBI:IPR019831, EBI:IPR001189, EBI:IPR019832, EBI:IPR019833 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:1.15.1.1 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
oxidation-reduction process	IEA: Inferred from Electronic Annotation with EBI:IPR019831, EBI:IPR001189, EBI:IPR019832, EBI:IPR019833 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0560 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
superoxide metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR019831, EBI:IPR001189, EBI:IPR019832, EBI:IPR019833 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrial matrix	IEA: Inferred from Electronic Annotation with EBI:SL-0170 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
mitochondrion	IEA: Inferred from Electronic Annotation with EBI:KW-0496 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).