This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2007-02-01    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
putrescine transmembrane transporter activity	IMP: Inferred from Mutant Phenotype Assigned on 2007-02-01 IDA: Inferred from Direct Assay Assigned on 2007-02-01	Uemura T, et al.  (2007) Polyamine Uptake by DUR3 and SAM3 in Saccharomyces cerevisiae. J Biol Chem 282(10):7733-41	SGD
spermidine transmembrane transporter activity	IMP: Inferred from Mutant Phenotype Assigned on 2007-02-01 IDA: Inferred from Direct Assay Assigned on 2007-02-01	Uemura T, et al.  (2007) Polyamine Uptake by DUR3 and SAM3 in Saccharomyces cerevisiae. J Biol Chem 282(10):7733-41	SGD
urea transmembrane transporter activity	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-10-03 IMP: Inferred from Mutant Phenotype Assigned on 2002-10-03	ElBerry HM, et al.  (1993) Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae. J Bacteriol 175(15):4688-98	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
putrescine transport	IMP: Inferred from Mutant Phenotype Assigned on 2007-02-01 IDA: Inferred from Direct Assay Assigned on 2007-02-01	Uemura T, et al.  (2007) Polyamine Uptake by DUR3 and SAM3 in Saccharomyces cerevisiae. J Biol Chem 282(10):7733-41	SGD
spermidine transport	IMP: Inferred from Mutant Phenotype Assigned on 2007-02-01 IDA: Inferred from Direct Assay Assigned on 2007-02-01	Uemura T, et al.  (2007) Polyamine Uptake by DUR3 and SAM3 in Saccharomyces cerevisiae. J Biol Chem 282(10):7733-41	SGD
urea catabolic process	IEP: Inferred from Expression Pattern Assigned on 2007-10-02	ElBerry HM, et al.  (1993) Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae. J Bacteriol 175(15):4688-98	SGD
urea transport	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-10-03 IMP: Inferred from Mutant Phenotype Assigned on 2002-10-03	ElBerry HM, et al.  (1993) Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae. J Bacteriol 175(15):4688-98	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
plasma membrane	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-10-03 IMP: Inferred from Mutant Phenotype Assigned on 2002-10-03	ElBerry HM, et al.  (1993) Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae. J Bacteriol 175(15):4688-98	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-02-01	Uemura T, et al.  (2007) Polyamine Uptake by DUR3 and SAM3 in Saccharomyces cerevisiae. J Biol Chem 282(10):7733-41	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	ISM: Inferred from Sequence Model Assigned on 2011-05-31	De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
transporter activity	IEA: Inferred from Electronic Annotation with EBI:IPR001734 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
transmembrane transport	IEA: Inferred from Electronic Annotation with EBI:IPR001734 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
transport	IEA: Inferred from Electronic Annotation with EBI:IPR019900, EBI:IPR001734 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0812 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:IPR019900, EBI:IPR001734 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0162 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).