This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2002-12-05    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
mRNA binding	IDA: Inferred from Direct Assay Assigned on 2011-02-01	Tsvetanova NG, et al.  (2010) Proteome-Wide Search Reveals Unexpected RNA-Binding Proteins in Saccharomyces cerevisiae.LID - e12671 [pii] PLoS One 5(9)	SGD
ubiquitin-specific protease activity	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Baker RT, et al.  (1992) Ubiquitin-specific proteases of Saccharomyces cerevisiae. Cloning of UBP2 and UBP3, and functional analysis of the UBP gene family. J Biol Chem 267(32):23364-75	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein deubiquitination	IMP: Inferred from Mutant Phenotype Assigned on 2008-08-22 IDA: Inferred from Direct Assay Assigned on 2008-08-22	Kvint K, et al.  (2008) Reversal of RNA Polymerase II Ubiquitylation by the Ubiquitin Protease Ubp3. Mol Cell 30(4):498-506	SGD
regulation of ER to Golgi vesicle-mediated transport	IMP: Inferred from Mutant Phenotype Assigned on 2010-09-30	Cohen M, et al.  (2003) Ubp3 requires a cofactor, Bre5, to specifically de-ubiquitinate the COPII protein, Sec23. Nat Cell Biol 5(7):661-7	SGD
regulation of response to osmotic stress	IMP: Inferred from Mutant Phenotype Assigned on 2011-11-01 IPI: Inferred from Physical Interaction with SGD:HOG1 Assigned on 2011-11-01	Sole C, et al.  (2011) Control of Ubp3 ubiquitin protease activity by the Hog1 SAPK modulates transcription upon osmostress.LID - 10.1038/emboj.2011.227 [doi] EMBO J ()	SGD
ribophagy	IMP: Inferred from Mutant Phenotype Assigned on 2008-06-30	Kraft C, et al.  (2008) Mature ribosomes are selectively degraded upon starvation by an autophagy pathway requiring the Ubp3p/Bre5p ubiquitin protease. Nat Cell Biol 10(5):602-10	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IC: Inferred By Curator from ubiquitin-specific protease activity Assigned on 2002-12-05	Baker RT, et al.  (1992) Ubiquitin-specific proteases of Saccharomyces cerevisiae. Cloning of UBP2 and UBP3, and functional analysis of the UBP gene family. J Biol Chem 267(32):23364-75	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for UBP3

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
cysteine-type peptidase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0788 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0378 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
peptidase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0645 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
ubiquitin thiolesterase activity	IEA: Inferred from Electronic Annotation with EBI:IPR018200, EBI:IPR001394 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
proteolysis	IEA: Inferred from Electronic Annotation with EBI:KW-0645 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
ubiquitin-dependent protein catabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR018200, EBI:IPR001394 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).