This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-12-15    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin binding	IDA: Inferred from Direct Assay Assigned on 2009-01-12	Sprouse RO, et al.  (2009) TATA-binding Protein Variants That Bypass the Requirement for Mot1 in Vivo. J Biol Chem 284(7):4525-35	SGD
DNA binding, bending	IDA: Inferred from Direct Assay Assigned on 2009-08-31	Horikoshi M, et al.  (1992) Transcription factor TFIID induces DNA bending upon binding to the TATA element. Proc Natl Acad Sci U S A 89(3):1060-4	SGD
RNA polymerase I transcription factor binding	IDA: Inferred from Direct Assay Assigned on 2011-10-19 IPI: Inferred from Physical Interaction with SGD:RRN9, SGD:RRN6, SGD:RRN11, SGD:RRN7 Assigned on 2011-10-19	Steffan JS, et al.  (1996) The role of TBP in rDNA transcription by RNA polymerase I in Saccharomyces cerevisiae: TBP is required for upstream activation factor-dependent recruitment of core factor. Genes Dev 10(20):2551-63	SGD
RNA polymerase I transcription factor recruiting transcription factor activity	IDA: Inferred from Direct Assay Assigned on 2011-10-19 IPI: Inferred from Physical Interaction with SGD:RRN6, SGD:RRN11, SGD:RRN7 Assigned on 2011-10-19	Steffan JS, et al.  (1996) The role of TBP in rDNA transcription by RNA polymerase I in Saccharomyces cerevisiae: TBP is required for upstream activation factor-dependent recruitment of core factor. Genes Dev 10(20):2551-63	SGD
RNA polymerase II activating transcription factor binding	IDA: Inferred from Direct Assay Assigned on 2011-12-15 IPI: Inferred from Physical Interaction with SGD:GAL4 Assigned on 2011-12-15	Klein J, et al.  (2003) Use of a genetically introduced cross-linker to identify interaction sites of acidic activators within native transcription factor IID and SAGA. J Biol Chem 278(9):6779-86	SGD
RNA polymerase II core promoter sequence-specific DNA binding	IDA: Inferred from Direct Assay Assigned on 2011-10-19	Horikoshi M, et al.  (1989) Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA box. Nature 341(6240):299-303	SGD
RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly	IC: Inferred By Curator from RNA polymerase II transcriptional preinitiation complex assembly, transcription factor TFIID complex, RNA polymerase II core promoter sequence-specific DNA binding Assigned on 2011-12-13	GO Annotation Working Group  (2011) Manual annotations that require more than one source of functional data to support the assignment of the associated GO term.	SGD
RNA polymerase III regulatory region DNA binding	IDA: Inferred from Direct Assay Assigned on 2011-07-21	Roberts S, et al.  (1996) Cloning and functional characterization of the gene encoding the TFIIIB90 subunit of RNA polymerase III transcription factor TFIIIB. J Biol Chem 271(25):14903-9	SGD
TFIIIB-type transcription factor activity	IC: Inferred By Curator from RNA polymerase III regulatory region DNA binding, transcription factor TFIIIB complex Assigned on 2011-10-19	GO Annotation Working Group  (2011) Manual annotations that require more than one source of functional data to support the assignment of the associated GO term.	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
RNA polymerase II transcriptional preinitiation complex assembly	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-04	Shen WC, et al.  (2003) Systematic analysis of essential yeast TAFs in genome-wide transcription and preinitiation complex assembly. EMBO J 22(13):3395-402	SGD
RNA polymerase III transcriptional preinitiation complex assembly	IDA: Inferred from Direct Assay Assigned on 2009-09-08	Huet J and Sentenac A  (1992) The TATA-binding protein participates in TFIIIB assembly on tRNA genes. Nucleic Acids Res 20(24):6451-4	SGD
transcription of nuclear large rRNA transcript from RNA polymerase I promoter	IMP: Inferred from Mutant Phenotype Assigned on 2011-10-19	Cormack BP and Struhl K  (1992) The TATA-binding protein is required for transcription by all three nuclear RNA polymerases in yeast cells. Cell 69(4):685-96	SGD
transposon integration	IDA: Inferred from Direct Assay Assigned on 2009-09-08	Yieh L, et al.  (2000) The Brf and TATA-binding protein subunits of the RNA polymerase III transcription factor IIIB mediate position-specific integration of the gypsy-like element, Ty3. J Biol Chem 275(38):29800-7	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IDA: Inferred from Direct Assay Assigned on 2002-03-19	Patterson GH, et al.  (1998) Quantitative imaging of TATA-binding protein in living yeast cells. Yeast 14(9):813-25	SGD
colocalizes_with RNA polymerase I core factor complex	IDA: Inferred from Direct Assay Assigned on 2009-08-25	Lalo D, et al.  (1996) RRN11 encodes the third subunit of the complex containing Rrn6p and Rrn7p that is essential for the initiation of rDNA transcription by yeast RNA polymerase I. J Biol Chem 271(35):21062-7	SGD
	IDA: Inferred from Direct Assay Assigned on 2009-08-25	Steffan JS, et al.  (1998) Interaction of TATA-binding protein with upstream activation factor is required for activated transcription of ribosomal DNA by RNA polymerase I in Saccharomyces cerevisiae in vivo. Mol Cell Biol 18(7):3752-61	SGD
colocalizes_with RNA polymerase I upstream activating factor complex	IDA: Inferred from Direct Assay Assigned on 2009-08-25	Steffan JS, et al.  (1998) Interaction of TATA-binding protein with upstream activation factor is required for activated transcription of ribosomal DNA by RNA polymerase I in Saccharomyces cerevisiae in vivo. Mol Cell Biol 18(7):3752-61	SGD
transcription factor TFIID complex	IDA: Inferred from Direct Assay Assigned on 2008-07-24	Sanders SL and Weil PA  (2000) Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex. J Biol Chem 275(18):13895-900	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-12-15	Auty R, et al.  (2004) Purification of active TFIID from Saccharomyces cerevisiae. Extensive promoter contacts and co-activator function. J Biol Chem 279(48):49973-81	SGD
transcription factor TFIIIB complex	IDA: Inferred from Direct Assay Assigned on 2009-08-31	Buratowski S and Zhou H  (1992) A suppressor of TBP mutations encodes an RNA polymerase III transcription factor with homology to TFIIB. Cell 71(2):221-30	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-11-28	Kassavetis GA, et al.  (1992) The role of the TATA-binding protein in the assembly and function of the multisubunit yeast RNA polymerase III transcription factor, TFIIIB. Cell 71(6):1055-64	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Molecular Function

High-throughput Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
sequence-specific DNA binding	IDA: Inferred from Direct Assay Assigned on 2009-02-05	Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR000814 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA-dependent transcription, initiation	IEA: Inferred from Electronic Annotation with EBI:IPR000814 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).