This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2007-05-24    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
FFAT motif binding	IMP: Inferred from Mutant Phenotype Assigned on 2007-04-09 IPI: Inferred from Physical Interaction with SGD:OPI1 Assigned on 2007-04-09	Loewen CJ, et al.  (2003) A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP. EMBO J 22(9):2025-35	SGD
phosphatidylinositol binding	IDA: Inferred from Direct Assay Assigned on 2007-11-30	Kagiwada S and Hashimoto M  (2007) The yeast VAP homolog Scs2p has a phosphoinositide-binding ability that is correlated with its activity. Biochem Biophys Res Commun 364(4):870-6	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin silencing at telomere	IGI: Inferred from Genetic Interaction with SGD:SIR2 Assigned on 2007-05-16	Cuperus G and Shore D  (2002) Restoration of silencing in Saccharomyces cerevisiae by tethering of a novel Sir2-interacting protein, Esc8. Genetics 162(2):633-45	SGD
	IGI: Inferred from Genetic Interaction with SGD:DUN1, SGD:MEC1 Assigned on 2007-05-17 IMP: Inferred from Mutant Phenotype Assigned on 2003-11-04	Craven RJ and Petes TD  (2001) The Saccharomyces cerevisiae suppressor of choline sensitivity (SCS2) gene is a multicopy Suppressor of mec1 telomeric silencing defects. Genetics 158(1):145-54	SGD
endoplasmic reticulum inheritance	IGI: Inferred from Genetic Interaction with SGD:ICE2 Assigned on 2008-09-30 IMP: Inferred from Mutant Phenotype Assigned on 2008-09-30	Loewen CJ, et al.  (2007) Inheritance of cortical ER in yeast is required for normal septin organization. J Cell Biol 179(3):467-83	SGD
endoplasmic reticulum membrane organization	IGI: Inferred from Genetic Interaction with SGD:IST2, SGD:TCB3, SGD:TCB2, SGD:TCB1, SGD:SCS22 Assigned on 2013-02-01	Manford AG, et al.  (2012) ER-to-Plasma Membrane Tethering Proteins Regulate Cell Signaling and ER Morphology. Dev Cell 23(6):1129-40	SGD
negative regulation of transcription factor import into nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2007-05-04 IPI: Inferred from Physical Interaction with SGD:OPI1 Assigned on 2007-05-04	Loewen CJ, et al.  (2003) A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP. EMBO J 22(9):2025-35	SGD
phospholipid biosynthetic process	IMP: Inferred from Mutant Phenotype Assigned on 2007-05-16	Kagiwada S and Zen R  (2003) Role of the yeast VAP homolog, Scs2p, in INO1 expression and phospholipid metabolism. J Biochem 133(4):515-22	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2007-05-15	Kagiwada S, et al.  (1998) The Saccharomyces cerevisiae SCS2 gene product, a homolog of a synaptobrevin-associated protein, is an integral membrane protein of the endoplasmic reticulum and is required for inositol metabolism. J Bacteriol 180(7):1700-8	SGD
	IGI: Inferred from Genetic Interaction with SGD:HAC1 Assigned on 2007-05-15	Nikawa J, et al.  (1995) Cloning and sequence of the SCS2 gene, which can suppress the defect of INO1 expression in an inositol auxotrophic mutant of Saccharomyces cerevisiae. J Biochem 118(1):39-45	SGD
regulation of intracellular lipid transport	IMP: Inferred from Mutant Phenotype Assigned on 2007-05-08	Loewen CJ, et al.  (2003) A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP. EMBO J 22(9):2025-35	SGD
regulation of phosphatidylinositol dephosphorylation	IGI: Inferred from Genetic Interaction with SGD:IST2, SGD:TCB3, SGD:TCB2, SGD:TCB1, SGD:SCS22 Assigned on 2013-02-01	Manford AG, et al.  (2012) ER-to-Plasma Membrane Tethering Proteins Regulate Cell Signaling and ER Morphology. Dev Cell 23(6):1129-40	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular bud tip	IDA: Inferred from Direct Assay Assigned on 2008-09-30	Loewen CJ, et al.  (2007) Inheritance of cortical ER in yeast is required for normal septin organization. J Cell Biol 179(3):467-83	SGD
endoplasmic reticulum	IDA: Inferred from Direct Assay Assigned on 2007-04-11	Loewen CJ, et al.  (2003) A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP. EMBO J 22(9):2025-35	SGD
integral to endoplasmic reticulum membrane	IDA: Inferred from Direct Assay Assigned on 2008-02-25	Kagiwada S, et al.  (1998) The Saccharomyces cerevisiae SCS2 gene product, a homolog of a synaptobrevin-associated protein, is an integral membrane protein of the endoplasmic reticulum and is required for inositol metabolism. J Bacteriol 180(7):1700-8	SGD
nuclear envelope	IDA: Inferred from Direct Assay Assigned on 2007-04-11	Loewen CJ, et al.  (2003) A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP. EMBO J 22(9):2025-35	SGD
nuclear membrane	IDA: Inferred from Direct Assay Assigned on 2007-05-04	Kagiwada S, et al.  (1998) The Saccharomyces cerevisiae SCS2 gene product, a homolog of a synaptobrevin-associated protein, is an integral membrane protein of the endoplasmic reticulum and is required for inositol metabolism. J Bacteriol 180(7):1700-8	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for SCS2

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
structural molecule activity	IEA: Inferred from Electronic Annotation with EBI:IPR000535 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
endoplasmic reticulum	IEA: Inferred from Electronic Annotation with EBI:KW-0256 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
endoplasmic reticulum membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0097 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
integral to membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0812 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nuclear membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0182 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).