This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-04-28    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
GTPase activity	IDA: Inferred from Direct Assay Assigned on 2010-03-24	Will E, et al.  (2001) Expression, purification, and biochemical properties of Ypt/Rab GTPase-activating proteins of Gyp family. Methods Enzymol 329:50-8	SGD
	IPI: Inferred from Physical Interaction Assigned on 2002-06-24	Yoo JS, et al.  (1999) Functional implications of genetic interactions between genes encoding small GTPases involved in vesicular transport in yeast. Mol Gen Genet 261(1):80-91	SGD
	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-06-24	Jedd G, et al.  (1997) Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment. J Cell Biol 137(3):563-80	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
early endosome to Golgi transport	IGI: Inferred from Genetic Interaction with SGD:YPT32 Assigned on 2010-12-10	Chen SH, et al.  (2005) Ypt31/32 GTPases and their novel F-box effector protein Rcy1 regulate protein recycling. Mol Biol Cell 16(1):178-92	SGD
exocytosis	IMP: Inferred from Mutant Phenotype Assigned on 2002-06-24	Jedd G, et al.  (1997) Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment. J Cell Biol 137(3):563-80	SGD
vesicle-mediated transport	IGI: Inferred from Genetic Interaction Assigned on 2002-06-24 IMP: Inferred from Mutant Phenotype Assigned on 2002-06-24	Jedd G, et al.  (1997) Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment. J Cell Biol 137(3):563-80	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
endosome	IDA: Inferred from Direct Assay Assigned on 2005-07-11	Chen SH, et al.  (2005) Ypt31/32 GTPases and their novel F-box effector protein Rcy1 regulate protein recycling. Mol Biol Cell 16(1):178-92	SGD
Golgi apparatus	IDA: Inferred from Direct Assay Assigned on 2005-07-11	Chen SH, et al.  (2005) Ypt31/32 GTPases and their novel F-box effector protein Rcy1 regulate protein recycling. Mol Biol Cell 16(1):178-92	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrial outer membrane	IDA: Inferred from Direct Assay Assigned on 2006-03-17	Zahedi RP, et al.  (2006) Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins. Mol Biol Cell 17(3):1436-50	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
GTP binding	IEA: Inferred from Electronic Annotation with EBI:IPR001806, EBI:IPR005225, EBI:IPR003579 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0342 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein transport	IEA: Inferred from Electronic Annotation with EBI:IPR003579 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
small GTPase mediated signal transduction	IEA: Inferred from Electronic Annotation with EBI:IPR001806, EBI:IPR003579 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
Golgi apparatus	IEA: Inferred from Electronic Annotation with EBI:KW-0333 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
Golgi membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0134 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).