This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2007-10-11    Molecular Function | Biological Process

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
phosphoacetylglucosamine mutase activity	ISS: Inferred from Sequence or structural Similarity Assigned on 2003-01-22 IMP: Inferred from Mutant Phenotype Assigned on 2003-01-22	Hofmann M, et al.  (1994) Characterization of the essential yeast gene encoding N-acetylglucosamine-phosphate mutase. Eur J Biochem 221(2):741-7	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
fungal-type cell wall chitin biosynthetic process	IMP: Inferred from Mutant Phenotype Assigned on 2007-10-11	Hofmann M, et al.  (1994) Characterization of the essential yeast gene encoding N-acetylglucosamine-phosphate mutase. Eur J Biochem 221(2):741-7	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2002-05-07	Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19	SGD
	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
intramolecular transferase activity, phosphotransferases	IEA: Inferred from Electronic Annotation with EBI:IPR005843, EBI:IPR005845, EBI:IPR016055, EBI:IPR005844 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
isomerase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0413 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
magnesium ion binding	IEA: Inferred from Electronic Annotation with EBI:IPR016066 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
phosphoacetylglucosamine mutase activity	IEA: Inferred from Electronic Annotation with EBI:IPR016657 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:5.4.2.3 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
phosphoglucomutase activity	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR22573_AN0 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
carbohydrate metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR005843, EBI:IPR005845, EBI:IPR016055, EBI:IPR005844 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
glucose 1-phosphate metabolic process	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR22573_AN0 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
UDP-N-acetylglucosamine biosynthetic process	IEA: Inferred from Electronic Annotation with UniPathway:UPA00113 Last updated 2013-03-02	UniProt-GOA  (2012) Gene Ontology annotation based on UniPathway vocabulary mapping.	UniPathway
	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR22573_AN318 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytosol	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR22573_AN0 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).