This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-02-04    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
serine-type peptidase activity	ISS: Inferred from Sequence or structural Similarity Assigned on 2003-02-04	Tatusov RL, et al.  (2000) The COG database: a tool for genome-scale analysis of protein functions and evolution. Nucleic Acids Res 28(1):33-6	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
ascospore wall assembly	IMP: Inferred from Mutant Phenotype Assigned on 2009-11-05	Suda Y, et al.  (2009) A screen for spore wall permeability mutants identifies a secreted protease required for proper spore wall assembly. PLoS One 4(9):e7184	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
ascospore wall	IDA: Inferred from Direct Assay Assigned on 2009-11-05	Suda Y, et al.  (2009) A screen for spore wall permeability mutants identifies a secreted protease required for proper spore wall assembly. PLoS One 4(9):e7184	SGD
nuclear envelope	IDA: Inferred from Direct Assay Assigned on 2009-11-05	Suda Y, et al.  (2009) A screen for spore wall permeability mutants identifies a secreted protease required for proper spore wall assembly. PLoS One 4(9):e7184	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for RRT12

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0378 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
identical protein binding	IEA: Inferred from Electronic Annotation with EBI:IPR010259 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
peptidase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0645 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
serine-type endopeptidase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000209, EBI:IPR010259 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10795_AN0 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
serine-type peptidase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0720 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
negative regulation of catalytic activity	IEA: Inferred from Electronic Annotation with EBI:IPR010259 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
proteolysis	IEA: Inferred from Electronic Annotation with EBI:IPR000209 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10795_AN631 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
	IEA: Inferred from Electronic Annotation with EBI:KW-0645 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
sporulation resulting in formation of a cellular spore	IEA: Inferred from Electronic Annotation with EBI:KW-0749 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
spore wall	IEA: Inferred from Electronic Annotation with EBI:SL-0254 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).