This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2006-11-17    Molecular Function | Biological Process

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
glucokinase activity	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Walsh RB, et al.  (1983) Cloning of genes that complement yeast hexokinase and glucokinase mutants. J Bacteriol 154(2):1002-4	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-06-09	Clifton D, et al.  (1993) Functional studies of yeast glucokinase. J Bacteriol 175(11):3289-94	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
glucose import	IGI: Inferred from Genetic Interaction with SGD:HXK2, SGD:HXK1 Assigned on 2006-11-17	Bisson LF and Fraenkel DG  (1983) Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 80(6):1730-4	SGD
glucose metabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2005-06-09	Bisson LF and Fraenkel DG  (1983) Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 80(6):1730-4	SGD
glycolysis	IDA: Inferred from Direct Assay Assigned on 2006-11-17	Clifton D, et al.  (1993) Functional studies of yeast glucokinase. J Bacteriol 175(11):3289-94	SGD
mannose metabolic process	IDA: Inferred from Direct Assay Assigned on 2006-11-17	Maitra PK  (1975) Glucokinase from yeast. Methods Enzymol 42:25-30	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytosol	IDA: Inferred from Direct Assay Assigned on 2006-11-17	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
plasma membrane	IDA: Inferred from Direct Assay Assigned on 2009-04-29	Delom F, et al.  (2006) The plasma membrane proteome of Saccharomyces cerevisiae and its response to the antifungal calcofluor. Proteomics 6(10):3029-39	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:IPR022672, EBI:IPR022673, EBI:IPR019807, EBI:IPR001312 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
glucokinase activity	IEA: Inferred from Electronic Annotation with IUBMB:2.7.1.2 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
hexokinase activity	IEA: Inferred from Electronic Annotation with EBI:IPR019807 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
kinase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0418 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
phosphotransferase activity, alcohol group as acceptor	IEA: Inferred from Electronic Annotation with EBI:IPR022672, EBI:IPR022673, EBI:IPR001312 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
transferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
carbohydrate metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR022672, EBI:IPR022673, EBI:IPR001312 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
glucose 6-phosphate metabolic process	IEA: Inferred from Electronic Annotation with IUBMB:2.7.1.2 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	GOC
glycolysis	IEA: Inferred from Electronic Annotation with EBI:IPR019807 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0324 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
phosphorylation	IEA: Inferred from Electronic Annotation with EBI:KW-0418 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).