This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2010-02-27    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein tyrosine/serine/threonine phosphatase activity	ISA: Inferred from Sequence Alignment with SGD:MSG5 Assigned on 2010-02-27 IDA: Inferred from Direct Assay Assigned on 2010-02-27	Ernsting BR and Dixon JE  (1997) The PPS1 gene of Saccharomyces cerevisiae codes for a dual specificity protein phosphatase with a role in the DNA synthesis phase of the cell cycle. J Biol Chem 272(14):9332-43	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
inactivation of MAPK activity	IMP: Inferred from Mutant Phenotype Assigned on 2010-02-27 IDA: Inferred from Direct Assay Assigned on 2010-02-27	Ernsting BR and Dixon JE  (1997) The PPS1 gene of Saccharomyces cerevisiae codes for a dual specificity protein phosphatase with a role in the DNA synthesis phase of the cell cycle. J Biol Chem 272(14):9332-43	SGD
nuclear cell cycle DNA replication	IMP: Inferred from Mutant Phenotype Assigned on 2013-04-25	Ernsting BR and Dixon JE  (1997) The PPS1 gene of Saccharomyces cerevisiae codes for a dual specificity protein phosphatase with a role in the DNA synthesis phase of the cell cycle. J Biol Chem 272(14):9332-43	SGD
protein dephosphorylation	IDA: Inferred from Direct Assay Assigned on 2004-06-07	Ernsting BR and Dixon JE  (1997) The PPS1 gene of Saccharomyces cerevisiae codes for a dual specificity protein phosphatase with a role in the DNA synthesis phase of the cell cycle. J Biol Chem 272(14):9332-43	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular_component unknown	ND: No Biological Data Available Assigned on 2002-09-30	SGD  (2002) Use of the ND evidence code for Gene Ontology (GO) terms in SGD ()	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for PPS1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0378 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
MAP kinase tyrosine/serine/threonine phosphatase activity	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10159_AN957 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
phosphatase activity	IEA: Inferred from Electronic Annotation with EBI:IPR016130, EBI:IPR000387 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
phosphoprotein phosphatase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0904 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with IUBMB:3.1.3.16 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
protein tyrosine phosphatase activity	IEA: Inferred from Electronic Annotation with EBI:IPR016130 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:3.1.3.48 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
protein tyrosine/serine/threonine phosphatase activity	IEA: Inferred from Electronic Annotation with EBI:IPR024950, EBI:IPR000340, EBI:IPR020422 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10159_AN957 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
cell cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
dephosphorylation	IEA: Inferred from Electronic Annotation with EBI:IPR016130, EBI:IPR000387 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
peptidyl-tyrosine dephosphorylation	IEA: Inferred from Electronic Annotation with EBI:IPR016130 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	GOC
	IEA: Inferred from Electronic Annotation with IUBMB:3.1.3.48 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	GOC
protein dephosphorylation	IEA: Inferred from Electronic Annotation with EBI:IPR024950, EBI:IPR000340, EBI:IPR020422 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10159_AN957 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytosol	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10159_AN957 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
nucleus	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10159_AN957 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).