This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2002-10-01    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
contributes_to ATPase activity	IDA: Inferred from Direct Assay Assigned on 2009-03-20	Vary JC Jr, et al.  (2003) Yeast Isw1p forms two separable complexes in vivo. Mol Cell Biol 23(1):80-91	SGD
contributes_to DNA binding	IDA: Inferred from Direct Assay Assigned on 2009-03-20	Vary JC Jr, et al.  (2003) Yeast Isw1p forms two separable complexes in vivo. Mol Cell Biol 23(1):80-91	SGD
contributes_to nucleosome binding	IDA: Inferred from Direct Assay Assigned on 2009-03-20	Vary JC Jr, et al.  (2003) Yeast Isw1p forms two separable complexes in vivo. Mol Cell Biol 23(1):80-91	SGD
rDNA binding	IDA: Inferred from Direct Assay Assigned on 2011-10-07	Jones HS, et al.  (2007) RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA. Nat Struct Mol Biol 14(2):123-30	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin remodeling	IGI: Inferred from Genetic Interaction Assigned on 2002-10-01 IMP: Inferred from Mutant Phenotype Assigned on 2002-10-01 IPI: Inferred from Physical Interaction Assigned on 2002-10-01	Kent NA, et al.  (2001) In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p. Genes Dev 15(5):619-26	SGD
DNA-dependent transcription, elongation	IMP: Inferred from Mutant Phenotype Assigned on 2005-11-07 IDA: Inferred from Direct Assay Assigned on 2005-11-07	Morillon A, et al.  (2005) Dynamic lysine methylation on histone H3 defines the regulatory phase of gene transcription. Mol Cell 18(6):723-34	SGD
heterochromatin maintenance involved in chromatin silencing	IGI: Inferred from Genetic Interaction with SGD:FUN30 Assigned on 2011-03-28 IMP: Inferred from Mutant Phenotype Assigned on 2011-03-28	Yu Q, et al.  (2011) Roles of chromatin remodeling factors in the formation and maintenance of heterochromatin structure. J Biol Chem 286(16):14659-69	SGD
nucleosome positioning	IGI: Inferred from Genetic Interaction with SGD:ISW2 Assigned on 2009-05-13 IMP: Inferred from Mutant Phenotype Assigned on 2009-05-13	Xella B, et al.  (2006) The ISWI and CHD1 chromatin remodelling activities influence ADH2 expression and chromatin organization. Mol Microbiol 59(5):1531-41	SGD
positive regulation of transcription from RNA polymerase II promoter	IMP: Inferred from Mutant Phenotype Assigned on 2009-05-13	Xella B, et al.  (2006) The ISWI and CHD1 chromatin remodelling activities influence ADH2 expression and chromatin organization. Mol Microbiol 59(5):1531-41	SGD
regulation of transcription by chromatin organization	IMP: Inferred from Mutant Phenotype Assigned on 2011-10-07	Xella B, et al.  (2006) The ISWI and CHD1 chromatin remodelling activities influence ADH2 expression and chromatin organization. Mol Microbiol 59(5):1531-41	SGD
regulation of transcriptional start site selection at RNA polymerase II promoter	IGI: Inferred from Genetic Interaction with SGD:CHD1 Assigned on 2011-10-11	Quan TK and Hartzog GA  (2010) Histone H3K4 and K36 Methylation, Chd1 and Rpd3S Oppose the Functions of Saccharomyces cerevisiae Spt4-Spt5 in Transcription. Genetics 184(2):321-34	SGD
termination of RNA polymerase I transcription	IGI: Inferred from Genetic Interaction with SGD:ISW2, SGD:CHD1 Assigned on 2011-10-07	Jones HS, et al.  (2007) RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA. Nat Struct Mol Biol 14(2):123-30	SGD
termination of RNA polymerase II transcription	IGI: Inferred from Genetic Interaction with SGD:ISW2, SGD:CHD1 Assigned on 2011-10-07	Alen C, et al.  (2002) A role for chromatin remodeling in transcriptional termination by RNA polymerase II. Mol Cell 10(6):1441-52	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
Isw1 complex	IPI: Inferred from Physical Interaction Assigned on 2004-10-20	Vary JC Jr, et al.  (2003) Yeast Isw1p forms two separable complexes in vivo. Mol Cell Biol 23(1):80-91	SGD
nucleolar chromatin	IDA: Inferred from Direct Assay Assigned on 2011-10-07	Jones HS, et al.  (2007) RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA. Nat Struct Mol Biol 14(2):123-30	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IDA: Inferred from Direct Assay Assigned on 2012-12-12	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:IPR015195, EBI:IPR001650, EBI:IPR000330 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
chromatin binding	IEA: Inferred from Electronic Annotation with EBI:IPR001005 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
DNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR009057, EBI:IPR000330 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
helicase activity	IEA: Inferred from Electronic Annotation with EBI:IPR001650 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0347 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0378 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	IEA: Inferred from Electronic Annotation with EBI:IPR015195 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleic acid binding	IEA: Inferred from Electronic Annotation with EBI:IPR015195, EBI:IPR001650 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleosome binding	IEA: Inferred from Electronic Annotation with EBI:IPR015194 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP-dependent chromatin remodeling	IEA: Inferred from Electronic Annotation with EBI:IPR015194 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
chromatin modification	IEA: Inferred from Electronic Annotation with EBI:KW-0156 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
chromatin remodeling	IEA: Inferred from Electronic Annotation with EBI:IPR015195 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:IPR015195 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).