This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2010-06-15    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
mRNA binding	IDA: Inferred from Direct Assay Assigned on 2010-06-15	Buu LM, et al.  (2004) The yeast RNA-binding protein Rbp1p modifies the stability of mitochondrial porin mRNA. J Biol Chem 279(1):453-62	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
3'-UTR-mediated mRNA destabilization	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-24 IDA: Inferred from Direct Assay Assigned on 2010-06-24	Buu LM, et al.  (2004) The yeast RNA-binding protein Rbp1p modifies the stability of mitochondrial porin mRNA. J Biol Chem 279(1):453-62	SGD
mitochondrion organization	IMP: Inferred from Mutant Phenotype Assigned on 2004-09-15	Buu LM, et al.  (2004) The yeast RNA-binding protein Rbp1p modifies the stability of mitochondrial porin mRNA. J Biol Chem 279(1):453-62	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasmic mRNA processing body	IDA: Inferred from Direct Assay Assigned on 2006-12-14	Jang LT, et al.  (2006) Determinants of Rbp1p localization in specific cytoplasmic mRNA-processing foci, P-bodies. J Biol Chem 281(39):29379-90	SGD
cytoplasmic stress granule	IDA: Inferred from Direct Assay Assigned on 2009-06-02	Grousl T, et al.  (2009) Robust heat shock induces eIF2{alpha}-phosphorylation-independent assembly of stress granules containing eIF3 and 40S ribosomal subunits in budding yeast, Saccharomyces cerevisiae. J Cell Sci 122(Pt 12):2078-88	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-11-19	Buchan JR, et al.  (2008) P bodies promote stress granule assembly in Saccharomyces cerevisiae. J Cell Biol 183(3):441-55	SGD
perinuclear region of cytoplasm	IDA: Inferred from Direct Assay Assigned on 2004-09-15	Buu LM, et al.  (2004) The yeast RNA-binding protein Rbp1p modifies the stability of mitochondrial porin mRNA. J Biol Chem 279(1):453-62	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2002-05-07	Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19	SGD
	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleic acid binding	IEA: Inferred from Electronic Annotation with EBI:IPR000504 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:IPR012677 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
RNA binding	IEA: Inferred from Electronic Annotation with EBI:KW-0694 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).