This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2006-04-21    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
hydrolase activity, acting on ester bonds	IDA: Inferred from Direct Assay Assigned on 2011-04-29	Debelyy MO, et al.  (2011) Involvement of the Saccharomyces cerevisiae hydrolase Ldh1p in lipid homeostasis. Eukaryot Cell 10(6):776-81	SGD
serine hydrolase activity	ISM: Inferred from Sequence Model Assigned on 2011-04-29 IDA: Inferred from Direct Assay Assigned on 2004-06-04	Baxter SM, et al.  (2004) Synergistic computational and experimental proteomics approaches for more accurate detection of active serine hydrolases in yeast. Mol Cell Proteomics 3(3):209-25	SGD
triglyceride lipase activity	IDA: Inferred from Direct Assay Assigned on 2011-04-29	Debelyy MO, et al.  (2011) Involvement of the Saccharomyces cerevisiae hydrolase Ldh1p in lipid homeostasis. Eukaryot Cell 10(6):776-81	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
lipid homeostasis	IMP: Inferred from Mutant Phenotype Assigned on 2011-04-29	Debelyy MO, et al.  (2011) Involvement of the Saccharomyces cerevisiae hydrolase Ldh1p in lipid homeostasis. Eukaryot Cell 10(6):776-81	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
lipid particle	IDA: Inferred from Direct Assay Assigned on 2011-05-20	Thoms S, et al.  (2011) The putative Saccharomyces cerevisiae hydrolase Ldh1p is localized to lipid droplets. Eukaryot Cell 10(6):770-5	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for LDH1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0378 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
retinyl-palmitate esterase activity	IEA: Inferred from Electronic Annotation with IUBMB:3.1.1.3 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
triglyceride lipase activity	IEA: Inferred from Electronic Annotation with IUBMB:3.1.1.3 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
lipid catabolic process	IEA: Inferred from Electronic Annotation with EBI:KW-0442 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
lipid metabolic process	IEA: Inferred from Electronic Annotation with EBI:KW-0443 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
lipid particle	IEA: Inferred from Electronic Annotation with EBI:SL-0154 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0551 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).