This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2006-10-17    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
proton-transporting ATPase activity, rotational mechanism	IMP: Inferred from Mutant Phenotype Assigned on 2006-10-16	Yamashiro CT, et al.  (1990) Role of vacuolar acidification in protein sorting and zymogen activation: a genetic analysis of the yeast vacuolar proton-translocating ATPase. Mol Cell Biol 10(7):3737-49	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular calcium ion homeostasis	IMP: Inferred from Mutant Phenotype Assigned on 2006-10-17	Forster C and Kane PM  (2000) Cytosolic Ca2+ homeostasis is a constitutive function of the V-ATPase in Saccharomyces cerevisiae. J Biol Chem 275(49):38245-53	SGD
transmembrane transport	IMP: Inferred from Mutant Phenotype Assigned on 2008-12-10	Yamashiro CT, et al.  (1990) Role of vacuolar acidification in protein sorting and zymogen activation: a genetic analysis of the yeast vacuolar proton-translocating ATPase. Mol Cell Biol 10(7):3737-49	SGD
vacuolar acidification	IMP: Inferred from Mutant Phenotype Assigned on 2008-09-26	Makrantoni V, et al.  (2007) A novel role for the yeast protein kinase Dbf2p in vacuolar H+-ATPase function and sorbic acid stress tolerance. Microbiology 153(Pt 12):4016-26	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2006-10-16	Yamashiro CT, et al.  (1990) Role of vacuolar acidification in protein sorting and zymogen activation: a genetic analysis of the yeast vacuolar proton-translocating ATPase. Mol Cell Biol 10(7):3737-49	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
fungal-type vacuole membrane	IDA: Inferred from Direct Assay Assigned on 2008-09-26	Makrantoni V, et al.  (2007) A novel role for the yeast protein kinase Dbf2p in vacuolar H+-ATPase function and sorbic acid stress tolerance. Microbiology 153(Pt 12):4016-26	SGD
vacuolar proton-transporting V-type ATPase, V1 domain	IDA: Inferred from Direct Assay Assigned on 2006-10-16	Uchida E, et al.  (1985) Purification and properties of H+-translocating, Mg2+-adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. J Biol Chem 260(2):1090-5	SGD
	IDA: Inferred from Direct Assay Assigned on 2006-10-16	Kane PM, et al.  (1989) Biochemical characterization of the yeast vacuolar H(+)-ATPase. J Biol Chem 264(32):19236-44	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2002-05-07	Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19	SGD
integral to membrane	ISM: Inferred from Sequence Model Assigned on 2011-05-31	De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:IPR020003, EBI:IPR000194 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0378 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	IEA: Inferred from Electronic Annotation with EBI:IPR000793, EBI:IPR005723 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP hydrolysis coupled proton transport	IEA: Inferred from Electronic Annotation with EBI:IPR000793, EBI:IPR005723 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
ATP metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR004100 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
ion transport	IEA: Inferred from Electronic Annotation with EBI:KW-0406 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
proton transport	IEA: Inferred from Electronic Annotation with EBI:IPR004100 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0375 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
proton-transporting two-sector ATPase complex, catalytic domain	IEA: Inferred from Electronic Annotation with EBI:IPR000793 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
proton-transporting V-type ATPase, V1 domain	IEA: Inferred from Electronic Annotation with EBI:IPR005723 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).