This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2007-02-12    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
L-aminoadipate-semialdehyde dehydrogenase activity	IMP: Inferred from Mutant Phenotype Assigned on 2007-02-09	Barnes DA and Thorner J  (1986) Genetic manipulation of Saccharomyces cerevisiae by use of the LYS2 gene. Mol Cell Biol 6(8):2828-38	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2007-02-09	Sinha AK and Bhattacharjee JK  (1971) Lysine biosynthesis in Saccharomyces. Conversion of -aminoadipate into -aminoadipic -semialdehyde. Biochem J 125(3):743-9	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-02-09	Ehmann DE, et al.  (1999) Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5. Biochemistry 38(19):6171-7	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
lysine biosynthetic process via aminoadipic acid	IMP: Inferred from Mutant Phenotype Assigned on 2007-02-09	Barnes DA and Thorner J  (1986) Genetic manipulation of Saccharomyces cerevisiae by use of the LYS2 gene. Mol Cell Biol 6(8):2828-38	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2007-02-09	Sinha AK and Bhattacharjee JK  (1971) Lysine biosynthesis in Saccharomyces. Conversion of -aminoadipate into -aminoadipic -semialdehyde. Biochem J 125(3):743-9	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-02-09	Ehmann DE, et al.  (1999) Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5. Biochemistry 38(19):6171-7	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	NAS: Non-traceable Author Statement Assigned on 2007-02-12	Bhattacharjee JK  (1985) alpha-Aminoadipate pathway for the biosynthesis of lysine in lower eukaryotes. Crit Rev Microbiol 12(2):131-51	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2007-02-12	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
catalytic activity	IEA: Inferred from Electronic Annotation with EBI:IPR000873 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
L-aminoadipate-semialdehyde dehydrogenase activity	IEA: Inferred from Electronic Annotation with EBI:IPR014397, EBI:IPR010080 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:1.2.1.31 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:IPR016040 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
oxidoreductase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0560 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
phosphopantetheine binding	IEA: Inferred from Electronic Annotation with EBI:IPR014397 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular amino acid biosynthetic process	IEA: Inferred from Electronic Annotation with EBI:KW-0028 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
lysine biosynthetic process	IEA: Inferred from Electronic Annotation with EBI:IPR014397 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0457 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
lysine biosynthetic process via aminoadipic acid	IEA: Inferred from Electronic Annotation with UniPathway:UPA00033 Last updated 2013-03-02	UniProt-GOA  (2012) Gene Ontology annotation based on UniPathway vocabulary mapping.	UniPathway
metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR000873 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
oxidation-reduction process	IEA: Inferred from Electronic Annotation with EBI:IPR014397 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0560 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).