This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2007-07-18    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
calcium ion binding	IDA: Inferred from Direct Assay Assigned on 2007-07-18	Davis TN, et al.  (1986) Isolation of the yeast calmodulin gene: calmodulin is an essential protein. Cell 47(3):423-31	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-05-03	Starovasnik MA, et al.  (1993) Similarities and differences between yeast and vertebrate calmodulin: an examination of the calcium-binding and structural properties of calmodulin from the yeast Saccharomyces cerevisiae. Biochemistry 32(13):3261-70	SGD
calcium-dependent protein binding	IDA: Inferred from Direct Assay Assigned on 2007-07-18	Cyert MS and Thorner J  (1992) Regulatory subunit (CNB1 gene product) of yeast Ca2+/calmodulin-dependent phosphoprotein phosphatases is required for adaptation to pheromone. Mol Cell Biol 12(8):3460-9	SGD
	IPI: Inferred from Physical Interaction with SGD:CNA1, SGD:CMK1 Assigned on 2007-07-18	Okano H, et al.  (1998) Importance of phenylalanine residues of yeast calmodulin for target binding and activation. J Biol Chem 273(41):26375-82	SGD
protein binding	IPI: Inferred from Physical Interaction with SGD:ARC35 Assigned on 2007-07-17	Schaerer-Brodbeck C and Riezman H  (2000) Functional interactions between the p35 subunit of the Arp2/3 complex and calmodulin in yeast. Mol Biol Cell 11(4):1113-27	SGD
	IPI: Inferred from Physical Interaction with SGD:MYO2 Assigned on 2007-07-16	Brockerhoff SE, et al.  (1994) The unconventional myosin, Myo2p, is a calmodulin target at sites of cell growth in Saccharomyces cerevisiae. J Cell Biol 124(3):315-23	SGD
	IPI: Inferred from Physical Interaction with SGD:SPC110 Assigned on 2007-07-16	Stirling DA, et al.  (1994) Interaction with calmodulin is required for the function of Spc110p, an essential component of the yeast spindle pole body. EMBO J 13(18):4329-42	SGD
	IPI: Inferred from Physical Interaction with SGD:MYO5 Assigned on 2007-07-17	Geli MI, et al.  (1998) Distinct functions of calmodulin are required for the uptake step of receptor-mediated endocytosis in yeast: the type I myosin Myo5p is one of the calmodulin targets. EMBO J 17(3):635-47	SGD
	IPI: Inferred from Physical Interaction with SGD:SPC110 Assigned on 2007-07-16	Geiser JR, et al.  (1993) The essential mitotic target of calmodulin is the 110-kilodalton component of the spindle pole body in Saccharomyces cerevisiae. Mol Cell Biol 13(12):7913-24	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell budding	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Ohya Y and Botstein D  (1994) Diverse essential functions revealed by complementing yeast calmodulin mutants. Science 263(5149):963-6	SGD
cytoskeleton organization	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Ohya Y and Botstein D  (1994) Diverse essential functions revealed by complementing yeast calmodulin mutants. Science 263(5149):963-6	SGD
karyogamy involved in conjugation with cellular fusion	IGI: Inferred from Genetic Interaction with SGD:SPC110 Assigned on 2007-07-18 IMP: Inferred from Mutant Phenotype Assigned on 2007-07-18	Okano H and Ohya Y  (2003) Binding of calmodulin to Nuf1p is required for karyogamy in Saccharomyces cerevisiae. Mol Genet Genomics 269(5):649-57	SGD
microautophagy	IMP: Inferred from Mutant Phenotype Assigned on 2007-07-13	Uttenweiler A, et al.  (2005) Microautophagic vacuole invagination requires calmodulin in a Ca2+-independent function. J Biol Chem 280(39):33289-97	SGD
NLS-bearing substrate import into nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2008-10-17	Hanover JA, et al.  (2007) The High Mobility Group Box Transcription Factor Nhp6Ap Enters the Nucleus by a Calmodulin-dependent, Ran-independent Pathway. J Biol Chem 282(46):33743-51	SGD
phosphatidylinositol biosynthetic process	IGI: Inferred from Genetic Interaction with SGD:MSS4 Assigned on 2007-07-18 IMP: Inferred from Mutant Phenotype Assigned on 2007-07-18	Desrivieres S, et al.  (2002) Calmodulin controls organization of the actin cytoskeleton via regulation of phosphatidylinositol (4,5)-bisphosphate synthesis in Saccharomyces cerevisiae. Biochem J 366(Pt 3):945-51	SGD
receptor-mediated endocytosis	IMP: Inferred from Mutant Phenotype Assigned on 2007-07-17	Geli MI, et al.  (1998) Distinct functions of calmodulin are required for the uptake step of receptor-mediated endocytosis in yeast: the type I myosin Myo5p is one of the calmodulin targets. EMBO J 17(3):635-47	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2007-07-16	Kubler E, et al.  (1994) Calcium-independent calmodulin requirement for endocytosis in yeast. EMBO J 13(23):5539-46	SGD
spindle pole body organization	IMP: Inferred from Mutant Phenotype Assigned on 2007-07-13	Sun GH, et al.  (1992) Mutations in yeast calmodulin cause defects in spindle pole body functions and nuclear integrity. J Cell Biol 119(6):1625-39	SGD
transcription factor import into nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2008-10-17	Hanover JA, et al.  (2007) The High Mobility Group Box Transcription Factor Nhp6Ap Enters the Nucleus by a Calmodulin-dependent, Ran-independent Pathway. J Biol Chem 282(46):33743-51	SGD
vacuole fusion, non-autophagic	IMP: Inferred from Mutant Phenotype Assigned on 2005-05-03 IDA: Inferred from Direct Assay Assigned on 2007-07-18	Peters C and Mayer A  (1998) Ca2+/calmodulin signals the completion of docking and triggers a late step of vacuole fusion. Nature 396(6711):575-80	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular bud neck	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Brockerhoff SE and Davis TN  (1992) Calmodulin concentrates at regions of cell growth in Saccharomyces cerevisiae. J Cell Biol 118(3):619-29	SGD
cellular bud tip	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Brockerhoff SE and Davis TN  (1992) Calmodulin concentrates at regions of cell growth in Saccharomyces cerevisiae. J Cell Biol 118(3):619-29	SGD
central plaque of spindle pole body	IDA: Inferred from Direct Assay Assigned on 2007-07-16	Spang A, et al.  (1996) The spacer protein Spc110p targets calmodulin to the central plaque of the yeast spindle pole body. J Cell Sci 109 ( Pt 9)():2229-37	SGD
	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Sundberg HA, et al.  (1996) Role of calmodulin and Spc110p interaction in the proper assembly of spindle pole body compenents. J Cell Biol 133(1):111-24	SGD
incipient cellular bud site	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Brockerhoff SE and Davis TN  (1992) Calmodulin concentrates at regions of cell growth in Saccharomyces cerevisiae. J Cell Biol 118(3):619-29	SGD
mating projection tip	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Brockerhoff SE and Davis TN  (1992) Calmodulin concentrates at regions of cell growth in Saccharomyces cerevisiae. J Cell Biol 118(3):619-29	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for CMD1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
calcium ion binding	IEA: Inferred from Electronic Annotation with EBI:IPR011992, EBI:IPR002048 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cytoskeleton	IEA: Inferred from Electronic Annotation with EBI:KW-0206 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
spindle pole body	IEA: Inferred from Electronic Annotation with EBI:SL-0252 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).