This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-09-30    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
contributes_to RNA polymerase II core promoter proximal region sequence-specific DNA binding	IDA: Inferred from Direct Assay Assigned on 2011-09-30	Madhani HD and Fink GR  (1997) Combinatorial control required for the specificity of yeast MAPK signaling. Science 275(5304):1314-7	SGD
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription	IDA: Inferred from Direct Assay Assigned on 2011-09-30	Madhani HD and Fink GR  (1997) Combinatorial control required for the specificity of yeast MAPK signaling. Science 275(5304):1314-7	SGD
RNA polymerase II transcription factor recruiting transcription factor activity	IGI: Inferred from Genetic Interaction with SGD:STE12 Assigned on 2011-09-30 IMP: Inferred from Mutant Phenotype Assigned on 2011-09-30	Chou S, et al.  (2006) Regulation of mating and filamentation genes by two distinct Ste12 complexes in Saccharomyces cerevisiae. Mol Cell Biol 26(13):4794-805	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
chronological cell aging	IMP: Inferred from Mutant Phenotype Assigned on 2011-09-30	Bruckner S, et al.  (2011) The TEA transcription factor Tec1 links TOR and MAPK pathways to coordinate yeast development. Genetics 189(2):479-94	SGD
invasive growth in response to glucose limitation	IMP: Inferred from Mutant Phenotype Assigned on 2009-02-27	Kohler T, et al.  (2002) Dual role of the Saccharomyces cerevisiae TEA/ATTS family transcription factor Tec1p in regulation of gene expression and cellular development. Eukaryot Cell 1(5):673-86	SGD
positive regulation of transcription from RNA polymerase II promoter in response to stress	IDA: Inferred from Direct Assay Assigned on 2011-09-30	Madhani HD and Fink GR  (1997) Combinatorial control required for the specificity of yeast MAPK signaling. Science 275(5304):1314-7	SGD
positive regulation of transposition, RNA-mediated	IMP: Inferred from Mutant Phenotype Assigned on 2011-09-30	Conte D Jr and Curcio MJ  (2000) Fus3 controls Ty1 transpositional dormancy through the invasive growth MAPK pathway. Mol Microbiol 35(2):415-27	SGD
pseudohyphal growth	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18 IDA: Inferred from Direct Assay Assigned on 2001-01-18	Madhani HD and Fink GR  (1997) Combinatorial control required for the specificity of yeast MAPK signaling. Science 275(5304):1314-7	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2005-02-18	Gavrias V, et al.  (1996) Saccharomyces cerevisiae TEC1 is required for pseudohyphal growth. Mol Microbiol 19(6):1255-63	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2006-06-28	Mosch HU and Fink GR  (1997) Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae. Genetics 145(3):671-84	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IDA: Inferred from Direct Assay Assigned on 2009-02-26	Shock TR, et al.  (2009) Hog1 mitogen-activated protein kinase (MAPK) interrupts signal transduction between the Kss1 MAPK and the Tec1 transcription factor to maintain pathway specificity. Eukaryot Cell 8(4):606-16	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Molecular Function

High-throughput Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
sequence-specific DNA binding	IDA: Inferred from Direct Assay Assigned on 2009-02-05	Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87	SGD
	IDA: Inferred from Direct Assay Assigned on 2009-03-09	Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA binding	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
sequence-specific DNA binding transcription factor activity	IEA: Inferred from Electronic Annotation with EBI:IPR000818 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:IPR000818 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:IPR000818 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).