This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-04-24    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
nuclear localization sequence binding	IPI: Inferred from Physical Interaction Assigned on 2002-01-25	Truant R, et al.  (1998) Identification and functional characterization of a novel nuclear localization signal present in the yeast Nab2 poly(A)+ RNA binding protein. Mol Cell Biol 18(3):1449-58	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IMP: Inferred from Mutant Phenotype Assigned on 2003-04-24	Asakawa K and Toh-e A  (2002) A defect of Kap104 alleviates the requirement of mitotic exit network gene functions in Saccharomyces cerevisiae. Genetics 162(4):1545-56	SGD
mRNA-binding (hnRNP) protein import into nucleus	IDA: Inferred from Direct Assay Assigned on 2002-01-25	Truant R, et al.  (1998) Identification and functional characterization of a novel nuclear localization signal present in the yeast Nab2 poly(A)+ RNA binding protein. Mol Cell Biol 18(3):1449-58	SGD
	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-01-25 IMP: Inferred from Mutant Phenotype Assigned on 2002-01-25 IPI: Inferred from Physical Interaction Assigned on 2002-01-25	Aitchison JD, et al.  (1996) Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins. Science 274(5287):624-7	SGD
protein import into nucleus	IDA: Inferred from Direct Assay Assigned on 2002-01-25	Truant R, et al.  (1998) Identification and functional characterization of a novel nuclear localization signal present in the yeast Nab2 poly(A)+ RNA binding protein. Mol Cell Biol 18(3):1449-58	SGD
	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-01-25 IMP: Inferred from Mutant Phenotype Assigned on 2002-01-25 IPI: Inferred from Physical Interaction Assigned on 2002-01-25	Aitchison JD, et al.  (1996) Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins. Science 274(5287):624-7	SGD
transcription factor import into nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2009-04-28 IPI: Inferred from Physical Interaction with SGD:TFG2 Assigned on 2009-04-28	Suel KE and Chook YM  (2009) Kap104p Imports the PY-NLS-containing Transcription Factor Tfg2p into the Nucleus. J Biol Chem 284(23):15416-24	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular bud neck	IDA: Inferred from Direct Assay Assigned on 2009-02-26	van den Bogaart G, et al.  (2009) Nuclear transport factor directs localization of protein synthesis during mitosis. Nat Cell Biol 11(3):350-6	SGD
cellular bud tip	IDA: Inferred from Direct Assay Assigned on 2009-02-26	van den Bogaart G, et al.  (2009) Nuclear transport factor directs localization of protein synthesis during mitosis. Nat Cell Biol 11(3):350-6	SGD
cytosol	IDA: Inferred from Direct Assay Assigned on 2002-01-25	Aitchison JD, et al.  (1996) Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins. Science 274(5287):624-7	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	ISM: Inferred from Sequence Model Assigned on 2011-05-31	De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein transport	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).