This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-11-14    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
glutathione-disulfide reductase activity	IDA: Inferred from Direct Assay Assigned on 2008-04-14	Mesecke N, et al.  (2008) A Novel Group of Glutaredoxins in the cis-Golgi Critical for Oxidative Stress Resistance. Mol Biol Cell 19(6):2673-80	SGD
protein homodimerization activity	IDA: Inferred from Direct Assay Assigned on 2008-01-08	Mesecke N, et al.  (2008) Two Novel Monothiol Glutaredoxins from Saccharomyces cerevisiae Provide Further Insight into Iron-Sulfur Cluster Binding, Oligomerization, and Enzymatic Activity of Glutaredoxins. Biochemistry 47(5):1452-63	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular response to oxidative stress	IEP: Inferred from Expression Pattern Assigned on 2008-11-14	Izquierdo A, et al.  (2008) Saccharomyces cerevisiae Grx6 and Grx7 are monothiol glutaredoxins associated with the early secretory pathway. Eukaryot Cell 7(8):1415-26	SGD
	IGI: Inferred from Genetic Interaction with SGD:GRX6 Assigned on 2008-11-14	Mesecke N, et al.  (2008) A Novel Group of Glutaredoxins in the cis-Golgi Critical for Oxidative Stress Resistance. Mol Biol Cell 19(6):2673-80	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cis-Golgi network	IDA: Inferred from Direct Assay Assigned on 2008-04-14	Mesecke N, et al.  (2008) A Novel Group of Glutaredoxins in the cis-Golgi Critical for Oxidative Stress Resistance. Mol Biol Cell 19(6):2673-80	SGD
Golgi apparatus	IDA: Inferred from Direct Assay Assigned on 2008-08-28	Izquierdo A, et al.  (2008) Saccharomyces cerevisiae Grx6 and Grx7 are monothiol glutaredoxins associated with the early secretory pathway. Eukaryot Cell 7(8):1415-26	SGD
Golgi lumen	IDA: Inferred from Direct Assay Assigned on 2008-04-14	Mesecke N, et al.  (2008) A Novel Group of Glutaredoxins in the cis-Golgi Critical for Oxidative Stress Resistance. Mol Biol Cell 19(6):2673-80	SGD
integral to membrane	IDA: Inferred from Direct Assay Assigned on 2008-08-28	Izquierdo A, et al.  (2008) Saccharomyces cerevisiae Grx6 and Grx7 are monothiol glutaredoxins associated with the early secretory pathway. Eukaryot Cell 7(8):1415-26	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
fungal-type vacuole	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
2 iron, 2 sulfur cluster binding	IEA: Inferred from Electronic Annotation with EBI:KW-0001 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
electron carrier activity	IEA: Inferred from Electronic Annotation with EBI:IPR002109 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
iron-sulfur cluster binding	IEA: Inferred from Electronic Annotation with EBI:KW-0411 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein disulfide oxidoreductase activity	IEA: Inferred from Electronic Annotation with EBI:IPR002109 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell redox homeostasis	IEA: Inferred from Electronic Annotation with EBI:IPR002109, EBI:IPR011899 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).