This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-05-13    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein tag	IMP: Inferred from Mutant Phenotype Assigned on 2013-01-09	Yu ZQ, et al.  (2012) Dual roles of Atg8-PE deconjugation by Atg4 in autophagy. Autophagy 8(6):883-92	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
autophagic vacuole assembly	IMP: Inferred from Mutant Phenotype Assigned on 2005-03-23	Kim J, et al.  (2001) Membrane recruitment of Aut7p in the autophagy and cytoplasm to vacuole targeting pathways requires Aut1p, Aut2p, and the autophagy conjugation complex. J Cell Biol 152(1):51-64	UniProtKB
cellular membrane fusion	IMP: Inferred from Mutant Phenotype Assigned on 2007-09-06 IDA: Inferred from Direct Assay Assigned on 2007-09-06	Nakatogawa H, et al.  (2007) Atg8, a Ubiquitin-like Protein Required for Autophagosome Formation, Mediates Membrane Tethering and Hemifusion. Cell 130(1):165-78	SGD
CVT pathway	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-13	Lang T, et al.  (1998) Aut2p and Aut7p, two novel microtubule-associated proteins are essential for delivery of autophagic vesicles to the vacuole. EMBO J 17(13):3597-607	SGD
ER to Golgi vesicle-mediated transport	IGI: Inferred from Genetic Interaction with SGD:BET1 Assigned on 2008-05-13 IMP: Inferred from Mutant Phenotype Assigned on 2008-05-13 IPI: Inferred from Physical Interaction with SGD:BET1 Assigned on 2008-05-13	Legesse-Miller A, et al.  (2000) Aut7p, a soluble autophagic factor, participates in multiple membrane trafficking processes. J Biol Chem 275(42):32966-73	SGD
mitochondrion degradation	IMP: Inferred from Mutant Phenotype Assigned on 2010-01-07	Kanki T, et al.  (2009) A genomic screen for yeast mutants defective in selective mitochondria autophagy. Mol Biol Cell 20(22):4730-8	SGD
piecemeal microautophagy of nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2008-08-28	Krick R, et al.  (2008) Piecemeal microautophagy of the nucleus requires the core macroautophagy genes. Mol Biol Cell 19(10):4492-505	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
autophagic vacuole	IDA: Inferred from Direct Assay Assigned on 2005-02-25	Kirisako T, et al.  (1999) Formation process of autophagosome is traced with Apg8/Aut7p in yeast. J Cell Biol 147(2):435-46	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-01-19	Kim J, et al.  (2001) Membrane recruitment of Aut7p in the autophagy and cytoplasm to vacuole targeting pathways requires Aut1p, Aut2p, and the autophagy conjugation complex. J Cell Biol 152(1):51-64	UniProtKB
	IDA: Inferred from Direct Assay Assigned on 2005-08-08	Meiling-Wesse K, et al.  (2005) Trs85 (Gsg1), a component of the TRAPP complexes, is required for the organization of the preautophagosomal structure during selective autophagy via the Cvt pathway. J Biol Chem 280(39):33669-78	SGD
cytosol	IDA: Inferred from Direct Assay Assigned on 2005-03-23	Kim J, et al.  (2001) Membrane recruitment of Aut7p in the autophagy and cytoplasm to vacuole targeting pathways requires Aut1p, Aut2p, and the autophagy conjugation complex. J Cell Biol 152(1):51-64	UniProtKB
extrinsic to membrane	IDA: Inferred from Direct Assay Assigned on 2008-05-13	Legesse-Miller A, et al.  (2000) Aut7p, a soluble autophagic factor, participates in multiple membrane trafficking processes. J Biol Chem 275(42):32966-73	SGD
fungal-type vacuole membrane	IDA: Inferred from Direct Assay Assigned on 2011-03-31 IPI: Inferred from Physical Interaction with SGD:NYV1 Assigned on 2011-03-31	Legesse-Miller A, et al.  (2000) Aut7p, a soluble autophagic factor, participates in multiple membrane trafficking processes. J Biol Chem 275(42):32966-73	SGD
pre-autophagosomal structure	IDA: Inferred from Direct Assay Assigned on 2008-05-13	Suzuki K, et al.  (2001) The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation. EMBO J 20(21):5971-81	SGD
	IDA: Inferred from Direct Assay Assigned on 2006-09-29	Amar N, et al.  (2006) Two newly identified sites in the ubiquitin-like protein Atg8 are essential for autophagy. EMBO Rep 7(6):635-42	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-04-30	Yorimitsu T and Klionsky DJ  (2005) Atg11 links cargo to the vesicle-forming machinery in the cytoplasm to vacuole targeting pathway. Mol Biol Cell 16(4):1593-605	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for ATG8

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
autophagy	IEA: Inferred from Electronic Annotation with EBI:KW-0072 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein transport	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
autophagic vacuole membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0022 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
CVT vesicle membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0081 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
cytoplasmic vesicle	IEA: Inferred from Electronic Annotation with EBI:KW-0968 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
vacuolar membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0271 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
vacuole	IEA: Inferred from Electronic Annotation with EBI:KW-0926 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).