This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2002-10-10    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ubiquitin binding	IDA: Inferred from Direct Assay Assigned on 2006-06-08	Swanson KA, et al.  (2006) Structural Basis for Monoubiquitin Recognition by the Ede1 UBA Domain. J Mol Biol 358(3):713-24	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
endocytosis	IMP: Inferred from Mutant Phenotype Assigned on 2001-02-06	Gagny B, et al.  (2000) A novel EH domain protein of Saccharomyces cerevisiae, Ede1p, involved in endocytosis. J Cell Sci 113 ( Pt 18)():3309-19	SGD
endoplasmic reticulum unfolded protein response	IMP: Inferred from Mutant Phenotype Assigned on 2007-12-05	Hancock LC, et al.  (2006) Genomic analysis of the Opi- phenotype. Genetics 173(2):621-34	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
actin cortical patch	IDA: Inferred from Direct Assay Assigned on 2001-02-06	Gagny B, et al.  (2000) A novel EH domain protein of Saccharomyces cerevisiae, Ede1p, involved in endocytosis. J Cell Sci 113 ( Pt 18)():3309-19	SGD
cellular bud neck	IDA: Inferred from Direct Assay Assigned on 2002-10-10	Ni L and Snyder M  (2001) A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 12(7):2147-70	SGD
cellular bud tip	IDA: Inferred from Direct Assay Assigned on 2002-10-10	Ni L and Snyder M  (2001) A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 12(7):2147-70	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mating projection tip	IDA: Inferred from Direct Assay Assigned on 2008-12-19	Narayanaswamy R, et al.  (2009) Systematic Definition of Protein Constituents along the Major Polarization Axis Reveals an Adaptive Reuse of the Polarization Machinery in Pheromone-Treated Budding Yeast. J Proteome Res 8(1):6-19	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
calcium ion binding	IEA: Inferred from Electronic Annotation with EBI:IPR002048, EBI:IPR011992 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
endocytosis	IEA: Inferred from Electronic Annotation with EBI:KW-0254 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).