This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2009-02-27    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP-dependent peptidase activity	IMP: Inferred from Mutant Phenotype Assigned on 2009-02-18	Suzuki CK, et al.  (1994) Requirement for the yeast gene LON in intramitochondrial proteolysis and maintenance of respiration. Science 264(5156):273-6	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-02-18	Van Dyck L, et al.  (1994) PIM1 encodes a mitochondrial ATP-dependent protease that is required for mitochondrial function in the yeast Saccharomyces cerevisiae. J Biol Chem 269(1):238-42	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
chaperone-mediated protein complex assembly	IGI: Inferred from Genetic Interaction with SGD:YTA12, SGD:AFG3 Assigned on 2009-02-26	Rep M, et al.  (1996) Promotion of mitochondrial membrane complex assembly by a proteolytically inactive yeast Lon. Science 274(5284):103-6	SGD
misfolded or incompletely synthesized protein catabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2009-02-25	von Janowsky B, et al.  (2005) Structural properties of substrate proteins determine their proteolysis by the mitochondrial AAA+ protease Pim1. Biol Chem 386(12):1307-17	SGD
	IGI: Inferred from Genetic Interaction with SGD:SSC1 Assigned on 2009-02-18 IMP: Inferred from Mutant Phenotype Assigned on 2009-02-18	Wagner I, et al.  (1994) Molecular chaperones cooperate with PIM1 protease in the degradation of misfolded proteins in mitochondria. EMBO J 13(21):5135-45	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrial matrix	IMP: Inferred from Mutant Phenotype Assigned on 2009-02-18	Suzuki CK, et al.  (1994) Requirement for the yeast gene LON in intramitochondrial proteolysis and maintenance of respiration. Science 264(5156):273-6	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Van Dyck L, et al.  (1994) PIM1 encodes a mitochondrial ATP-dependent protease that is required for mitochondrial function in the yeast Saccharomyces cerevisiae. J Biol Chem 269(1):238-42	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:IPR003959, EBI:IPR004815 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10046_AN0 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
ATP-dependent peptidase activity	IEA: Inferred from Electronic Annotation with EBI:IPR003111, EBI:IPR008269, EBI:IPR008268, EBI:IPR004815 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
DNA binding	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0378 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitochondrial light strand promoter anti-sense binding	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10046_AN2 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
nucleoside-triphosphatase activity	IEA: Inferred from Electronic Annotation with EBI:IPR003593 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:IPR003593 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
peptidase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0645 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
serine-type endopeptidase activity	IEA: Inferred from Electronic Annotation with EBI:IPR008269, EBI:IPR008268, EBI:IPR027065 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
serine-type peptidase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0720 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
single-stranded DNA binding	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10046_AN2 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
single-stranded RNA binding	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10046_AN2 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular response to oxidative stress	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10046_AN2 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
misfolded or incompletely synthesized protein catabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR027065 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
mitochondrion organization	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10046_AN2 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
oxidation-dependent protein catabolic process	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10046_AN1 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
protein homooligomerization	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10046_AN2 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
proteolysis	IEA: Inferred from Electronic Annotation with EBI:IPR003111, EBI:IPR008269, EBI:IPR008268, EBI:IPR004815 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0645 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
response to hypoxia	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10046_AN2 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrial matrix	IEA: Inferred from Electronic Annotation with EBI:SL-0170 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
mitochondrial nucleoid	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10046_AN2 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
mitochondrion	IEA: Inferred from Electronic Annotation with EBI:KW-0496 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).