This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-06-06    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
contributes_to glycine dehydrogenase (decarboxylating) activity	ISA: Inferred from Sequence Alignment with EBI:P16048 Assigned on 2008-06-06 IMP: Inferred from Mutant Phenotype Assigned on 2002-11-01	Nagarajan L and Storms RK  (1997) Molecular characterization of GCV3, the Saccharomyces cerevisiae gene coding for the glycine cleavage system hydrogen carrier protein. J Biol Chem 272(7):4444-50	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
glycine catabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2008-06-06	Nagarajan L and Storms RK  (1997) Molecular characterization of GCV3, the Saccharomyces cerevisiae gene coding for the glycine cleavage system hydrogen carrier protein. J Biol Chem 272(7):4444-50	SGD
one-carbon metabolic process	IGI: Inferred from Genetic Interaction with SGD:SHM2 Assigned on 2008-06-06	Piper MD, et al.  (2000) Regulation of the balance of one-carbon metabolism in Saccharomyces cerevisiae. J Biol Chem 275(40):30987-95	SGD
protein lipoylation	IMP: Inferred from Mutant Phenotype Assigned on 2009-07-06	Schonauer MS, et al.  (2009) Lipoic Acid synthesis and attachment in yeast mitochondria. J Biol Chem 284(35):23234-42	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
glycine cleavage complex	ISA: Inferred from Sequence Alignment with EBI:P16048 Assigned on 2008-06-06	Nagarajan L and Storms RK  (1997) Molecular characterization of GCV3, the Saccharomyces cerevisiae gene coding for the glycine cleavage system hydrogen carrier protein. J Biol Chem 272(7):4444-50	SGD
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2009-07-06	Schonauer MS, et al.  (2009) Lipoic Acid synthesis and attachment in yeast mitochondria. J Biol Chem 284(35):23234-42	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-09-28	Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
glycine catabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR002930 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
glycine decarboxylation via glycine cleavage system	IEA: Inferred from Electronic Annotation with EBI:IPR017453 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
glycine cleavage complex	IEA: Inferred from Electronic Annotation with EBI:IPR002930 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
mitochondrion	IEA: Inferred from Electronic Annotation with EBI:SL-0173 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0496 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).