This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-10-13    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
contributes_to RNA binding	IC: Inferred By Curator from mRNA cleavage and polyadenylation specificity factor complex Assigned on 2005-02-18	Zhao J, et al.  (1999) Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor. Mol Cell Biol 19(11):7733-40	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
mRNA cleavage	IMP: Inferred from Mutant Phenotype Assigned on 2004-09-28	Zhao J, et al.  (1999) Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor. Mol Cell Biol 19(11):7733-40	SGD
mRNA polyadenylation	IMP: Inferred from Mutant Phenotype Assigned on 2004-09-28	Preker PJ, et al.  (1997) A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor. EMBO J 16(15):4727-37	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2004-09-28	Zhao J, et al.  (1999) Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor. Mol Cell Biol 19(11):7733-40	SGD
termination of RNA polymerase II transcription, exosome-dependent	IPI: Inferred from Physical Interaction Assigned on 2003-10-13	Nedea E, et al.  (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10	SGD
termination of RNA polymerase II transcription, poly(A)-coupled	IPI: Inferred from Physical Interaction Assigned on 2003-10-13	Nedea E, et al.  (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10	SGD
tRNA processing	IMP: Inferred from Mutant Phenotype Assigned on 2002-11-25	O'Connor JP and Peebles CL  (1992) PTA1, an essential gene of Saccharomyces cerevisiae affecting pre-tRNA processing. Mol Cell Biol 12(9):3843-56	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mRNA cleavage and polyadenylation specificity factor complex	IPI: Inferred from Physical Interaction Assigned on 2004-09-28	Zhao J, et al.  (1999) Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor. Mol Cell Biol 19(11):7733-40	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-04-05	Nedea E, et al.  (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10	SGD
mRNA cleavage factor complex	IPI: Inferred from Physical Interaction Assigned on 2002-04-10	Zhao J, et al.  (1997) Cleavage factor II of Saccharomyces cerevisiae contains homologues to subunits of the mammalian Cleavage/ polyadenylation specificity factor and exhibits sequence-specific, ATP-dependent interaction with precursor RNA. J Biol Chem 272(16):10831-8	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for PTA1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
mRNA processing	IEA: Inferred from Electronic Annotation with EBI:KW-0507 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
tRNA processing	IEA: Inferred from Electronic Annotation with EBI:KW-0819 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).