This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-10-28    Molecular Function | Biological Process

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
cystathionine gamma-lyase activity	IMP: Inferred from Mutant Phenotype Assigned on 2009-04-01	Ono BI, et al.  (1999) Cysteine biosynthesis in Saccharomyces cerevisiae: a new outlook on pathway and regulation. Yeast 15(13):1365-75	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-03-24	Cherest H, et al.  (1993) Cysteine biosynthesis in Saccharomyces cerevisiae occurs through the transsulfuration pathway which has been built up by enzyme recruitment. J Bacteriol 175(17):5366-74	SGD
	IDA: Inferred from Direct Assay Assigned on 2002-11-13	Ono B, et al.  (1992) Cloning and characterization of the CYS3 (CYI1) gene of Saccharomyces cerevisiae. J Bacteriol 174(10):3339-47	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cysteine biosynthetic process	IMP: Inferred from Mutant Phenotype Assigned on 2009-03-24	Cherest H, et al.  (1993) Cysteine biosynthesis in Saccharomyces cerevisiae occurs through the transsulfuration pathway which has been built up by enzyme recruitment. J Bacteriol 175(17):5366-74	SGD
cysteine biosynthetic process via cystathionine	IMP: Inferred from Mutant Phenotype Assigned on 2009-04-01	Ono BI, et al.  (1999) Cysteine biosynthesis in Saccharomyces cerevisiae: a new outlook on pathway and regulation. Yeast 15(13):1365-75	SGD
transsulfuration	IMP: Inferred from Mutant Phenotype Assigned on 2009-03-24	Cherest H, et al.  (1993) Cysteine biosynthesis in Saccharomyces cerevisiae occurs through the transsulfuration pathway which has been built up by enzyme recruitment. J Bacteriol 175(17):5366-74	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2013-01-20	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD
	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2012-12-12	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
catalytic activity	IEA: Inferred from Electronic Annotation with EBI:IPR015421, EBI:IPR015422 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
cystathionine gamma-lyase activity	IEA: Inferred from Electronic Annotation with IUBMB:4.4.1.1 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
L-cysteine desulfhydrase activity	IEA: Inferred from Electronic Annotation with IUBMB:4.4.1.1 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
L-cystine L-cysteine-lyase (deaminating)	IEA: Inferred from Electronic Annotation with IUBMB:4.4.1.1 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
lyase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0456 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
pyridoxal phosphate binding	IEA: Inferred from Electronic Annotation with EBI:IPR000277, EBI:IPR015422, EBI:IPR015421 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular amino acid biosynthetic process	IEA: Inferred from Electronic Annotation with EBI:KW-0028 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cysteine biosynthetic process	IEA: Inferred from Electronic Annotation with UniPathway:UPA00136 Last updated 2013-03-02	UniProt-GOA  (2012) Gene Ontology annotation based on UniPathway vocabulary mapping.	UniPathway
	IEA: Inferred from Electronic Annotation with EBI:KW-0198 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).