This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-12-02    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
molecular_function unknown	ND: No Biological Data Available Assigned on 2002-11-19	SGD  (2002) Use of the ND evidence code for Gene Ontology (GO) terms in SGD ()	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
establishment of mitochondrion localization	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-02	Boldogh IR, et al.  (2003) A protein complex containing Mdm10p, Mdm12p, and Mmm1p links mitochondrial membranes and DNA to the cytoskeleton-based segregation machinery. Mol Biol Cell 14(11):4618-27	SGD
mitochondrial outer membrane translocase complex assembly	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-02 IPI: Inferred from Physical Interaction with SGD:TOM22 Assigned on 2011-12-02	Thornton N, et al.  (2010) Two modular forms of the mitochondrial sorting and assembly machinery are involved in biogenesis of alpha-helical outer membrane proteins. J Mol Biol 396(3):540-9	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-02	Meisinger C, et al.  (2004) The mitochondrial morphology protein Mdm10 functions in assembly of the preprotein translocase of the outer membrane. Dev Cell 7(1):61-71	SGD
mitochondrion organization	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-02	Kornmann B, et al.  (2009) An ER-mitochondria tethering complex revealed by a synthetic biology screen. Science 325(5939):477-81	SGD
phospholipid transport	IGI: Inferred from Genetic Interaction with SGD:CRD1 Assigned on 2009-12-01	Kornmann B, et al.  (2009) An ER-mitochondria tethering complex revealed by a synthetic biology screen. Science 325(5939):477-81	SGD
protein import into mitochondrial outer membrane	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-17	Meisinger C, et al.  (2007) The morphology proteins Mdm12/Mmm1 function in the major beta-barrel assembly pathway of mitochondria. EMBO J 26(9):2229-39	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
ERMES complex	IPI: Inferred from Physical Interaction with SGD:MDM12 Assigned on 2007-07-27	Boldogh IR, et al.  (2003) A protein complex containing Mdm10p, Mdm12p, and Mmm1p links mitochondrial membranes and DNA to the cytoskeleton-based segregation machinery. Mol Biol Cell 14(11):4618-27	SGD
	IPI: Inferred from Physical Interaction with SGD:SAM35, SGD:SAM37, SGD:SAM50 Assigned on 2007-08-17	Meisinger C, et al.  (2007) The morphology proteins Mdm12/Mmm1 function in the major beta-barrel assembly pathway of mitochondria. EMBO J 26(9):2229-39	SGD
mitochondrial sorting and assembly machinery complex	IPI: Inferred from Physical Interaction with SGD:MMM1, SGD:MDM12 Assigned on 2007-08-17	Meisinger C, et al.  (2007) The morphology proteins Mdm12/Mmm1 function in the major beta-barrel assembly pathway of mitochondria. EMBO J 26(9):2229-39	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrial outer membrane	IDA: Inferred from Direct Assay Assigned on 2006-03-17	Zahedi RP, et al.  (2006) Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins. Mol Biol Cell 17(3):1436-50	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Cellular Component

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0812 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitochondrial outer membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0172 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-1000 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitochondrion	IEA: Inferred from Electronic Annotation with EBI:KW-0496 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).