This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2012-01-13    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
contributes_to DNA binding, bending	IDA: Inferred from Direct Assay Assigned on 2012-01-05	Leveillard T, et al.  (1991) Saccharomyces cerevisiae transcription factors IIIB and IIIC bend the DNA of a tRNA(Gln) gene. J Biol Chem 266(8):5162-8	SGD
contributes_to RNA polymerase III type 1 promoter sequence-specific DNA binding	IDA: Inferred from Direct Assay Assigned on 2012-01-05	Braun BR, et al.  (1989) Multiple states of protein-DNA interaction in the assembly of transcription complexes on Saccharomyces cerevisiae 5S ribosomal RNA genes. Proc Natl Acad Sci U S A 86(8):2530-4	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-01-05	Kassavetis GA, et al.  (1990) S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factors. Cell 60(2):235-45	SGD
contributes_to RNA polymerase III type 1 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity	IDA: Inferred from Direct Assay Assigned on 2012-01-05	Braun BR, et al.  (1989) Multiple states of protein-DNA interaction in the assembly of transcription complexes on Saccharomyces cerevisiae 5S ribosomal RNA genes. Proc Natl Acad Sci U S A 86(8):2530-4	SGD
contributes_to RNA polymerase III type 2 promoter sequence-specific DNA binding	IDA: Inferred from Direct Assay Assigned on 2012-01-05	Kassavetis GA, et al.  (1990) S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factors. Cell 60(2):235-45	SGD
contributes_to RNA polymerase III type 2 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity	IDA: Inferred from Direct Assay Assigned on 2012-01-05	Kassavetis GA, et al.  (1989) Transcription factor IIIB generates extended DNA interactions in RNA polymerase III transcription complexes on tRNA genes. Mol Cell Biol 9(6):2551-66	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
5S class rRNA transcription from RNA polymerase III type 1 promoter	IMP: Inferred from Mutant Phenotype Assigned on 2012-01-13	Lefebvre O, et al.  (1994) A mutation in the largest subunit of yeast TFIIIC affects tRNA and 5 S RNA synthesis. Identification of two classes of suppressors. J Biol Chem 269(37):23374-81	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-01-13	Felts SJ, et al.  (1990) Transcription factor requirements for in vitro formation of transcriptionally competent 5S rRNA gene chromatin. Mol Cell Biol 10(5):2390-401	SGD
protein localization to chromatin	IMP: Inferred from Mutant Phenotype Assigned on 2010-02-24	D'Ambrosio C, et al.  (2008) Identification of cis-acting sites for condensin loading onto budding yeast chromosomes. Genes Dev 22(16):2215-27	SGD
transcription from a RNA polymerase III hybrid type promoter	IDA: Inferred from Direct Assay Assigned on 2012-01-13	Burnol AF, et al.  (1993) TFIIIC relieves repression of U6 snRNA transcription by chromatin. Nature 362(6419):475-7	SGD
transcription from RNA polymerase III type 2 promoter	IDA: Inferred from Direct Assay Assigned on 2012-01-13	Baker RE, et al.  (1986) Effects of tRNATyr point mutations on the binding of yeast RNA polymerase III transcription factor C. J Biol Chem 261(12):5275-82	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-01-05	Felts SJ, et al.  (1990) Transcription factor requirements for in vitro formation of transcriptionally competent 5S rRNA gene chromatin. Mol Cell Biol 10(5):2390-401	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
colocalizes_with nuclear condensin complex	IDA: Inferred from Direct Assay Assigned on 2009-04-07	D'Ambrosio C, et al.  (2008) Identification of cis-acting sites for condensin loading onto budding yeast chromosomes. Genes Dev 22(16):2215-27	SGD
transcription factor TFIIIC complex	IDA: Inferred from Direct Assay Assigned on 2010-05-20	Parsons MC and Weil PA  (1990) Purification and characterization of Saccharomyces cerevisiae transcription factor TFIIIC. Polypeptide composition defined with polyclonal antibodies. J Biol Chem 265(9):5095-103	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-05	Lefebvre O, et al.  (1992) TFC3: gene encoding the B-block binding subunit of the yeast transcription factor IIIC. Proc Natl Acad Sci U S A 89(21):10512-6	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-01-05	Braun BR, et al.  (1992) Topography of transcription factor complexes on the Saccharomyces cerevisiae 5 S RNA gene. J Mol Biol 228(4):1063-77	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-01-05	Leveillard T, et al.  (1991) Saccharomyces cerevisiae transcription factors IIIB and IIIC bend the DNA of a tRNA(Gln) gene. J Biol Chem 266(8):5162-8	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-09-28	Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA binding	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IEA: Inferred from Electronic Annotation with EBI:SL-0173 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0496 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).