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Yeast Genome Sequence is Released - April 24, 1996


The Complete DNA Sequence of S. cerevisiae


The complete sequence of the yeast Saccharomyces cerevisiae strain S288C has been determined in an international collaboration of more than 100 laboratories.

The sequence data can be copied from the following ftp sites:


      America   genome-ftp.stanford.edu (directory /yeast/sequence/genomic_sequence/)


The contributing consortia are:

                                complete chromosomes      partial chromosomes
EU (Goffeau)                    II, III, VII, X, XI       IV, XII, XVI
                                XIV,XV
St. Louis (Johnston)            VIII                      IV, XVI
Sanger (Barrell)                IX, XIII                  IV, XVI
McGill (Bussey)                 I                         XVI
Stanford (Davis)                V                         IV
Riken (Murakami)                VI



CHROMOSOME          DNA Coordinator     Length              Release   

I               H. Bussey, Montreal       230,195          1995 (modif. 4-1996)
II              H. Feldmann, Munich       813,137        8-1994 (modif. 4-1996)
III             S. Oliver, Manchester     315,344       12-1991 (modif. 4-1996)
IV (I)          C. Jacq, Paris        
IV (II)         B. Barrell, Cambridge   
IV (III)        M. Johnston, St. Louis
IV (IV)         R. Davis, Stanford                                    
                                        1,522,191        4-1996
V               R. Davis, Stanford        574,860          1995 (modif. 4-1996)
VI              Y. Murakami, Tokyo        270,148          1995
VII             H. Tettelin, Louvain    1,090,936        4-1996
VIII            M. Johnston, St. Louis    562,638        8-1994
IX              B. Barell, Cambridge      439,885          1995
X               F. Gallibert, Rouen       745,443       10-1995 (modif. 4-1996)
XI              B. Dujon, Paris           666,448        4-1994
XII (left)      J. Hoheisel, Heidelberg 
XII (right)     M. Johnston, St. Louis
                                        1,078,171        4-1996
XIII            B. Barell, Cambridge      924,430          1995
XIV             P. Philippsen, Basel      784,328        4-1996
XV              B. Dujon, Paris         1,091,282        4-1996
XVI (I)         A. Goffeau, Louvain               
XVI (II)        H. Bussey, Montreal           
XVI (IIa)       R. Davis, Stanford                                    
XVI (III)       B. Barrell, Cambridge   
XVI (IV)        M. Johnston, St. Louis

                                          948,061        4-1996

mitochondrial genome (as compiled by de Zamaroczy M. and Bernardi G.)
                Length:    78,520 nt
__________________________________________
Total:                 12,136,020 nt
                       12,057,500 nt (without mitochondrial genome)

The yeast genome contains several regions with extensive repeated sequences.
These repeats have not been sequenced in their entirety but at least
one mostly two copies have been included in the final sequences.

Missing repeated sequences: 
~ 100 copies of rDNA repeat(chrXII) each ~9,137 nt    = 913,700 nt
2 copies of the ENA2 (chrIV) repeat ~3,885 nt         =   7,770 nt           
~ 10 copies of the CUP1 repeat (chrVIII) ~ 1,998 nt   =  19,980 nt
~ 3-5 copies of Y' elements each ~6,700 nt
  (chrIV telR 2-3 copies; chrXII telR 1-2 copies)     =  26,800 nt
~ 750 nt of telomeric sequence of chrVI               =     750 nt
___________________________________________________________________
Total of missing repeated sequences:                    969,000 nt


The total genome of S.c. S288C therefore contains:     12,136,020 nt
                                                      +   969,000 nt
                                                     _______________
                                                       13,105,020 nt


NOTE: The strain S288C lacks some of the gene families found in brewery and
      other industrial strains such as the MEL, MAL and SUC gene families and
      may be others.


Sequence data released before April 1996 are annotated and the systematic
nomenclature has been assigned. Due to the priority of the rapid release
of the data to the scientific community, the assignment of the systematic
nomenclature for open reading frames has not yet been completed for all
16 chromosomes. In collaboration with the consortia involved in this project
these nomenclature will subsequently be added to the information on the WWW
pages (see below) and finally included in tables on the FTP sites. (Please do
NOT start doing your own nomenclature to avoid unnecessary confusion!)

Detailed information on open reading frames, genetic elements, and the
graphical display by the GSG (Genome Sequence Graph) are available on
the WWW:
 
    http://mips.gsf.de

Reference information is linked to the S. cerevisiae page for each
chromosome. Information on the web includes homology listings and graphical
display of the chromosomes. A detailed classification of open reading
frames is in progress.

H.W. Mewes & K. Kleine
Max-Planck-Inst. f. Biochemie
MIPS
82152 Martinsried, Germany
Tel.: +49 89 8578 2657
FAX : +49 89 8578 2655
email: mewes@mips.embnet.org
       kleine@mips.embnet.org


=========================================================================

The data were assembled (EU part) and edited by MIPS (Max-Planck-Inst. f. Biochemie,
Martinsried, Germany). We are greatful for comments, corrections of sequence
data, and any contribution to the annotation of the data (to be submitted
to : zollner@mips.embnet.org ). 

Acknowledgement:

The ftp-directories contain the proper reference for each chromosome.
The following MIPS staff members have contributed to the data evaluation:
                    
Yeast sequence processing:
K. Kleine, K. Albermann, J. Hani, H. Tettelin (Louvain), A. Zollner

Informatics:
A. Gleissner, C. Harris (WWW), K. Heumann (HPT/GSG), S. Liebl (XChromo), 
H.W. Mewes (coordinator), A. Maierl, P. Zaccharia

Protein Sequence Databank (annotation):
G. Fobo, F. Pfeiffer

MIPS is supported by the Max-Planck-Gesellschaft, the Forschungszentrum
f. Umwelt u. Gesundheit (GSF), the European Commission, and the BMBF

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